SDS-PAGE: Difference between revisions

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===Solutions for various polyacrylamide gels (SDS-PAGE)===
===Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)===
  Chemical 8% 10% 12%
  Chemical     8%     10%     12%
  Water 3ml
  Water         4.74  4.0    3.3  ml
30% AA(*)    2.66  3.3    4.0  ml
LOWER buffer  2.5    2.5    2.5  ml

Revision as of 03:08, 15 May 2007

Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)

Chemical      8%     10%     12%
Water         4.74   4.0     3.3  ml
30% AA(*)     2.66   3.3     4.0  ml
LOWER buffer  2.5    2.5     2.5  ml