SDS-PAGE: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(SDS link) |
||
| (One intermediate revision by one other user not shown) | |||
| Line 6: | Line 6: | ||
30% AA(*) 2.66 3.3 4.0 ml | 30% AA(*) 2.66 3.3 4.0 ml | ||
LOWER buffer 2.5 2.5 2.5 ml | LOWER buffer 2.5 2.5 2.5 ml | ||
20% SDS 50 50 50 ul | 20% [[SDS]] 50 50 50 ul | ||
10% APS 200 200 200 ul | 10% APS 200 200 200 ul | ||
TEMED 8.2 8.2 8.2 ul | TEMED 8.2 8.2 8.2 ul | ||
| Line 17: | Line 17: | ||
30% AA(*) 0.67 ml | 30% AA(*) 0.67 ml | ||
UPPER buffer 0.65 ml | UPPER buffer 0.65 ml | ||
20% SDS 20 ul | 20% [[SDS]] 20 ul | ||
10% APS 125 ul | 10% APS 125 ul | ||
TEMED 6 ul | TEMED 6 ul | ||
| Line 29: | Line 29: | ||
AA = acrylamide | AA = acrylamide | ||
SDS = sodium dodecylsulfate | [[SDS]] = sodium dodecylsulfate | ||
APS = ammonium persulfate | APS = ammonium persulfate | ||
| Line 35: | Line 35: | ||
[http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html SDS-PAGE "Hall of Shame"] - examples of common failures with debugging tips. | [http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html SDS-PAGE "Hall of Shame"] - examples of common failures with debugging tips. | ||
[[Category:Material]] | [[Category:Material]] [[Category:PAGE]] | ||
Revision as of 07:48, 8 April 2009
Running (lower) gel: 10 ml (2 MINIgels)
Chemical 8% 10% 12% Water 4.74 4.0 3.3 ml 30% AA(*) 2.66 3.3 4.0 ml LOWER buffer 2.5 2.5 2.5 ml 20% SDS 50 50 50 ul 10% APS 200 200 200 ul TEMED 8.2 8.2 8.2 ul
Stacking (upper) gel: 4 ml (2 MINIgels)
Chemical 4% Water 2.65 ml 30% AA(*) 0.67 ml UPPER buffer 0.65 ml 20% SDS 20 ul 10% APS 125 ul TEMED 6 ul
Buffers
LOWER buffer: 1M Tris base, pH 8.8 UPPER buffer: 1M Tris base, pH 6.8
Abbreviations
AA = acrylamide SDS = sodium dodecylsulfate APS = ammonium persulfate
Notes
SDS-PAGE "Hall of Shame" - examples of common failures with debugging tips.
