SDS-PAGE - Revision history

Jakob Suckale: SDS link

Jakob Suckale — Wed, 08 Apr 2009 14:48:47 GMT

SDS link

Jason R. Kelly — Wed, 01 Oct 2008 05:40:42 GMT

Notes

←Older revision		Revision as of 05:40, 1 October 2008
Line 35:		Line 35:
	[http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html SDS-PAGE "Hall of Shame"] - examples of common failures with debugging tips.		[http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html SDS-PAGE "Hall of Shame"] - examples of common failures with debugging tips.

-	[[Category:Material]] ~~and~~ [[Category:PAGE]]	+	[[Category:Material]] [[Category:PAGE]]

Jason R. Kelly — Wed, 01 Oct 2008 05:40:16 GMT

←Older revision		Revision as of 05:40, 1 October 2008
Line 31:		Line 31:
	SDS = sodium dodecylsulfate		SDS = sodium dodecylsulfate
	APS = ammonium persulfate		APS = ammonium persulfate
		+
		+	==Notes==
		+	[http://www.ruf.rice.edu/~bioslabs/studies/sds-page/sdsgoofs.html SDS-PAGE "Hall of Shame"] - examples of common failures with debugging tips.

	[[Category:Material]] and [[Category:PAGE]]		[[Category:Material]] and [[Category:PAGE]]

Torsten Waldminghaus — Tue, 11 Mar 2008 21:20:01 GMT

added tags

←Older revision		Revision as of 21:20, 11 March 2008
Line 31:		Line 31:
	SDS = sodium dodecylsulfate		SDS = sodium dodecylsulfate
	APS = ammonium persulfate		APS = ammonium persulfate
		+
		+	[[Category:Material]] and [[Category:PAGE]]

Mcebe — Wed, 16 May 2007 08:39:39 GMT

Abbreviations

Mcebe — Tue, 15 May 2007 10:17:52 GMT

Stacking (upper) gel: 4 ml (2 MINIgels)

Mcebe — Tue, 15 May 2007 10:13:41 GMT

SDS-PAGE solutions

←Older revision		Revision as of 10:13, 15 May 2007
Line 1:		Line 1:
-	===~~Solutions for 10 ml~~ (~~MINIgel~~) ~~of various polyacrylamide gels~~ (~~SDS-PAGE~~)===	+	===Running (lower) gel: 10 ml (2 MINIgels)===
		+
	Chemical 8% 10% 12%		Chemical 8% 10% 12%

Line 5:		Line 6:
	30% AA(*) 2.66 3.3 4.0 ml		30% AA(*) 2.66 3.3 4.0 ml
	LOWER buffer 2.5 2.5 2.5 ml		LOWER buffer 2.5 2.5 2.5 ml
		+	20% SDS 50 50 50 ul
		+	10% APS 200 200 200 ul
		+	TEMED 8.2 8.2 8.2 ul
		+
		+	===Stacking (upper) gel: 4 ml (2 MINIgels)===
		+
		+	Chemical 4%
		+
		+	Water 2.65 ml
		+	30% AA(*) 0.67 ml
		+	UPPER buffer 0.65 ml
		+	20% SDS 20 ul
		+	10% APS 125 ul
		+	TEMED 6 ul

Mcebe — Tue, 15 May 2007 10:09:03 GMT

Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)

←Older revision		Revision as of 10:09, 15 May 2007
Line 1:		Line 1:
	===Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)===		===Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)===
	Chemical 8% 10% 12%		Chemical 8% 10% 12%
-	~~----~~	+
	Water 4.74 4.0 3.3 ml		Water 4.74 4.0 3.3 ml
	30% AA(*) 2.66 3.3 4.0 ml		30% AA(*) 2.66 3.3 4.0 ml
	LOWER buffer 2.5 2.5 2.5 ml		LOWER buffer 2.5 2.5 2.5 ml

Mcebe — Tue, 15 May 2007 10:08:39 GMT

Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)

←Older revision		Revision as of 10:08, 15 May 2007
Line 1:		Line 1:
	===Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)===		===Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)===
	Chemical 8% 10% 12%		Chemical 8% 10% 12%
		+	----
	Water 4.74 4.0 3.3 ml		Water 4.74 4.0 3.3 ml
	30% AA(*) 2.66 3.3 4.0 ml		30% AA(*) 2.66 3.3 4.0 ml
	LOWER buffer 2.5 2.5 2.5 ml		LOWER buffer 2.5 2.5 2.5 ml

Mcebe — Tue, 15 May 2007 10:08:20 GMT

Solutions for various polyacrylamide gels (SDS-PAGE)

←Older revision		Revision as of 10:08, 15 May 2007
Line 1:		Line 1:
-	===Solutions for various polyacrylamide gels (SDS-PAGE)===	+	===Solutions for 10 ml (MINIgel) of various polyacrylamide gels (SDS-PAGE)===
-	Chemical 8% 10% 12%	+	Chemical 8% 10% 12%
-	Water ~~3ml~~	+	Water 4.74 4.0 3.3 ml
		+	30% AA(*) 2.66 3.3 4.0 ml
		+	LOWER buffer 2.5 2.5 2.5 ml