SEED/2008/Day 7: Difference between revisions
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(New page: == Morning == * dilute cells to log-phase growth * different amounts of induction with IPTG * test F2620 with different amount of AHL + IPTG * sequencing analysis ** anything that's incorr...) |
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Revision as of 12:40, 22 February 2008
Morning
- dilute cells to log-phase growth
- different amounts of induction with IPTG
- test F2620 with different amount of AHL + IPTG
- sequencing analysis
- anything that's incorrect: pick another blue/green colony per plate for growth and templiphi in strips
Afternoon
- beta-gal assay
- glycerol/set up sequencing as before for additional colonies
Instructor Post-prep
- grow up cultures of Top10 w/ and w/o a lacZ-alpha expressing plasmid
- grow up cells expressing F2620.lacZa
