SEED/2008/Day 7: Difference between revisions
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== Morning == | == Morning == | ||
* Discuss sequencing technologies | |||
* Review labs so far | |||
== Afternoon == | |||
* pick 1 blue/green colony per plate for growth and templiphi in strips | * pick 1 blue/green colony per plate for growth and templiphi in strips | ||
** pick 4 colonies per group | |||
** 2.5ul sample buffer per tube | |||
** pick colony and dip in tube | |||
** 3m@95C to break open cells | |||
** Add 2.5ul reaction buffer + 0.1ul enzyme mix | |||
* incubate templiphi at 30C overnight | * incubate templiphi at 30C overnight | ||
* Class project mini-presentations/discussions | |||
* | |||
== Instructor Post-prep == | == Instructor Post-prep == | ||
* heat kill templiphi | * heat kill templiphi | ||
* transfer 2ul of templiphi into sequencing mixes | * transfer 2ul of templiphi into sequencing mixes with VF2 | ||
* Send out for sequencing | * Send out for sequencing | ||
Latest revision as of 11:06, 13 April 2008
Morning
- Discuss sequencing technologies
- Review labs so far
Afternoon
- pick 1 blue/green colony per plate for growth and templiphi in strips
- pick 4 colonies per group
- 2.5ul sample buffer per tube
- pick colony and dip in tube
- 3m@95C to break open cells
- Add 2.5ul reaction buffer + 0.1ul enzyme mix
- incubate templiphi at 30C overnight
- Class project mini-presentations/discussions
Instructor Post-prep
- heat kill templiphi
- transfer 2ul of templiphi into sequencing mixes with VF2
- Send out for sequencing
