SEED/2009/Schedule: Difference between revisions

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! Agenda
! Agenda
! Homework
! Homework
! Lab Protocol
|-
|-
| [[../Day 1/]]
| [[../Day 1/]]
| Feb. 7
| Feb. 6
| safety training, [[Media:7.02-training-lab.pdf|training lab]]
| safety training, [[Media:7.02-training-lab.pdf|training lab]]
|  
|  
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** Data Analysis
** Data Analysis
** Pre-Lab
** Pre-Lab
| Lab Day #1: Serial Dilutions [[Media:SEED_pipette.pdf|pdf]]  [[Media:SEED_pipette.doc|doc]]
|-
|-
| [[../Day 2/]]
| [[../Day 2/]]
| Feb. 21
| Feb. 20
| SynBio Overview, sterile technique, colony streaking, antibiotic resistance
| SynBio Overview, sterile technique, colony streaking, antibiotic resistance
|
|
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** Read [http://en.wikipedia.org/wiki/Subcloning subcloning] on wikipedia
** Read [http://en.wikipedia.org/wiki/Subcloning subcloning] on wikipedia
* Pre-Lab: [[Media:PreLab2-2009.doc|doc]] [[Media:PreLab2-2009.pdf|pdf]]
* Pre-Lab: [[Media:PreLab2-2009.doc|doc]] [[Media:PreLab2-2009.pdf|pdf]]
* Quiz 1: [[Media:SEED Quiz1 2009.doc|doc]]
* Quiz 1: [[Media:SEED Quiz1 2009.doc|doc]] [[Media:SEED Quiz1 2009.pdf|pdf]]
| Lab Day #2: Plate Streaking [[Media:SEED_plate_streaking.pdf|pdf]] [[Media:SEED_plate_streaking.doc|doc]]  
|-
|-
| [[../Day 3/]]
| [[../Day 3/]]
| Feb. 28
| Feb. 27
| miniprep, digest
| miniprep, digest
|
|
* Homework #3: [[Media:SEED_HW3-2009.doc|doc]] [[Media:SEED_HW3-2009.pdf|pdf]]
* Homework #3: [[Media:SEED_HW3-2009.doc|doc]] [[Media:SEED_HW3-2009.pdf|pdf]]
* Pre-Lab #3: [[Media:SEED_PreLab3-2009.doc|doc]] [[Media:SEED_PreLab3-2009.pdf|pdf]]
* Pre-Lab #3: [[Media:SEED_PreLab3-2009.doc|doc]] [[Media:SEED_PreLab3-2009.pdf|pdf]]
|
* Lab Day #3: Plasmid Mini-Prep [[Media:SEED_mini_prep.pdf|pdf]] [[Media:SEED_mini_prep.doc|doc]]
* Restriction Digest [[Media:SEED_digest.pdf|pdf]] [[Media:SEED_digest.doc|doc]]
|-
|-
| [[../Day 4/]]
| [[../Day 4/]]
| Mar. 7
| Mar. 6
| PCR and gel
| PCR and gel
|
|
* Homework #4: [[Media:SEED_HW4-2009.doc|doc]] [[Media:SEED_HW4-2009.pdf|pdf]]
* Homework #4: [[Media:SEED_HW4-2009.doc|doc]] [[Media:SEED_HW4-2009.pdf|pdf]]
|
* Lab Day #4: PCR [[Media:SEED_PCR.pdf|pdf]] [[Media:SEED_PCR.doc|doc]]
* Gel Electrophoresis [[Media:SEED_gel_electrophoresis.pdf|pdf]] [[Media:SEED_gel_electrophoresis.doc|doc]]
|-
|-
| [[../Day 5/]]
| [[../Day 5/]]
| Mar. 14
| Mar. 13
| gel purification: [[Media:SEED_Gel_pur-2009.pdf|pdf]] [[Media:SEED_Gel_pur-2009.docx|docx]]
| gel purification: [[Media:SEED_Gel_pur-2009.pdf|pdf]] [[Media:SEED_Gel_pur-2009.docx|docx]]
|
|
* Homework #5: [[Media:SEED_HW5-2009.doc|docx]] [[Media:SEED_HW5-2009.pdf|pdf]]
* Homework #5: [[Media:SEED_HW5-2009.doc|docx]] [[Media:SEED_HW5-2009.pdf|pdf]]
* Quiz 2: [[Media:SEED Quiz2 2009.doc|doc]]
* Quiz 2: [[Media:SEED Quiz2 2009.doc|doc]] [[Media:SEED Quiz2 2009.pdf|pdf]]
| Lab Day #5: Gel Purification [[Media:SEED_gel_purification.pdf|pdf]] [[Media:SEED_gel_purification.doc|doc]]
|-
|-
| [[../Day 6/]]
| [[../Day 6/]]
| Mar. 21
| Mar. 20
| ligation, transformation
| ligation, transformation
|
|
* Pre-Lab #6: [http://www.zerobio.com/videos/sanger.html sequencing]
* Pre-Lab #6: [http://www.zerobio.com/videos/sanger.html sequencing]
* Quiz 3: [[Media:SEED Quiz3 2009.doc|doc]]
* Quiz 3: [[Media:SEED Quiz3 2009.doc|doc]] [[Media:SEED Quiz3 2009.pdf|pdf]]
| Lab Day #6: Ligation [[Media:SEED_ligation.pdf|pdf]] [[Media:SEED_ligation.doc|doc]]
|-
|-
| [[../Day 7/]]
| [[../Day 7/]]
| Mar. 28
| Apr. 3
| prep for sequencing, glycerol  
| prep for sequencing, glycerol  
final project notes:  
final project notes:  
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* Homework #7: [[Media:SEED_HW7-2009.doc|doc]] [[Media:SEED_HW7-2009.pdf|pdf]]
* Homework #7: [[Media:SEED_HW7-2009.doc|doc]] [[Media:SEED_HW7-2009.pdf|pdf]]
* Pre-Lab #5: [[Media:SEED_PL5-2009.doc|doc]] [[Media:SEED_PL5-2009.pdf|pdf]]
* Pre-Lab #5: [[Media:SEED_PL5-2009.doc|doc]] [[Media:SEED_PL5-2009.pdf|pdf]]
* Quiz 4: [[Media:SEED Quiz4 2009.doc|doc]]
* Quiz 4: [[Media:SEED Quiz4 2009.doc|doc]] [[Media:SEED Quiz4 2009.pdf|pdf]]
| Lab Day #7: Transformation [[Media:SEED_transformation.pdf|pdf]] [[Media:SEED_transformation.doc|doc]]
|-
|-
| [[../Day 8/]]
| [[../Day 8/]]
| Apr. 4
| Apr. 10
| β-gal assays, growth curves, viable cell titer, analyze sequencing
| β-gal assays, growth curves, viable cell titer, analyze sequencing
|
|
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* [[../Sequencing|Sequence results]]
* [[../Sequencing|Sequence results]]
* [[../Sequences|Sequences]]
* [[../Sequences|Sequences]]
| Lab Day #8: Beta-Galactosidase: Part I [[Media:SEED_beta_gal.pdf|pdf]] [[Media:SEED_beta_gal.doc|doc]]
|-
|-
| [[../Day 9/]]
| [[../Day 9/]]
| Apr. 18
| Apr. 24
| device characterization and composability
| device characterization and composability
|
|
* Homework #9: TBA
* Homework #9: TBA
| Lab Day #9: Beta-Galactosidase: Part II [[Media:SEED_inducibile_promoter.pdf|pdf]] [[Media:SEED_inducibile_promoter.doc|doc]]
|-
|-
| [[../Day 10/]]
| [[../Day 10/]]
| May 2
| May 1
| data analysis, finish projects
| data analysis, finish projects
|
|
* Final: TBA
* Final: TBA
| Final Lab: pdf
|}
|}
</div>
</div>
</div>
</div>

Latest revision as of 10:55, 2 February 2010

http://web.mit.edu/seed/images/seed.gif

[[../Overview/]] of the main lab project

Class Date Agenda Homework Lab Protocol
[[../Day 1/]] Feb. 6 safety training, training lab
  • Homework #1: doc pdf
    • read comic
    • iGEM Questions
    • Ideas (3-5) for what you want to engineer biology to do
    • Training lab questions
    • Data Analysis
    • Pre-Lab
 Lab Day #1: Serial Dilutions pdf doc
[[../Day 2/]] Feb. 20 SynBio Overview, sterile technique, colony streaking, antibiotic resistance Lab Day #2: Plate Streaking pdf doc
[[../Day 3/]] Feb. 27 miniprep, digest
  • Lab Day #3: Plasmid Mini-Prep pdf doc
  • Restriction Digest pdf doc
[[../Day 4/]] Mar. 6 PCR and gel
[[../Day 5/]] Mar. 13 gel purification: pdf docx Lab Day #5: Gel Purification pdf doc
[[../Day 6/]] Mar. 20 ligation, transformation Lab Day #6: Ligation pdf doc
[[../Day 7/]] Apr. 3 prep for sequencing, glycerol

final project notes:

Lab Day #7: Transformation pdf doc
[[../Day 8/]] Apr. 10 β-gal assays, growth curves, viable cell titer, analyze sequencing
  • Homework #8: TBA
  • [[../Sequencing|Sequence results]]
  • [[../Sequences|Sequences]]
 Lab Day #8: Beta-Galactosidase: Part I pdf doc
[[../Day 9/]] Apr. 24 device characterization and composability
  • Homework #9: TBA
 Lab Day #9: Beta-Galactosidase: Part II pdf doc
[[../Day 10/]] May 1 data analysis, finish projects
  • Final: TBA
 Final Lab: pdf
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