SEED/2010/Day 7: Difference between revisions
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(New page: == Morning == * Discuss sequencing technologies * Review labs so far == Afternoon == * pick 1 blue/green colony per plate for growth and templiphi in strips ** pick 4 colonies per group *...) |
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Latest revision as of 10:39, 2 February 2010
Morning
- Discuss sequencing technologies
- Review labs so far
Afternoon
- pick 1 blue/green colony per plate for growth and templiphi in strips
- pick 4 colonies per group
- 2.5ul sample buffer per tube
- pick colony and dip in tube
- 3m@95C to break open cells
- Add 2.5ul reaction buffer + 0.1ul enzyme mix
- incubate templiphi at 30C overnight
- Class project mini-presentations/discussions
Instructor Post-prep
- heat kill templiphi
- transfer 2ul of templiphi into sequencing mixes with VF2
- Send out for sequencing
