SEED/2011/Schedule: Difference between revisions

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** Training lab questions
** Training lab questions
** Data Analysis
** Data Analysis
| Lab Day #1: Serial Dilutions [[Media:SEED_pipette.pdf|pdf]] [[Media:SEED_pipette.doc|doc]]  
| Lab Day #1: Serial Dilutions  [[Media:SEED_pipette.doc|doc]]  
|-
|-
| [[../Day 2/]]
| [[../Day 2/]]
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| SynBio Overview, sterile technique, colony streaking, antibiotic resistance
| SynBio Overview, sterile technique, colony streaking, antibiotic resistance
|
|
* Homework #2:  
* Homework #2: [[Media:SEED_HW2-2011.docx|docx]] [[Media:SEED_HW2-2011.pdf|pdf]]
* Pre-Lab: [[Media:SEED_student.session3-PL.docx|docx]] [[Media:SEED_student.session3-PL.pdf|pdf]]
| Lab Day #2: Plate Streaking [[Media:SEED_plate_streaking.pdf|pdf]] [[Media:SEED_plate_streaking.doc|doc]]  
| Lab Day #2: Plate Streaking [[Media:SEED_plate_streaking.pdf|pdf]] [[Media:SEED_plate_streaking.doc|doc]]  
|-
|-
| [[../Day 3/]]
| [[../Day 3/]]
| Feb. 26
| Feb. 26
| miniprep, digest
| cloning, PCR
|
|
* Homework #3:  
* Homework #3: [[Media:SEED_HW3a-2011.docx|docx]] [[Media:SEED_HW3a-2011.pdf|pdf]]
* Pre-Lab #3:  
* Pre-Lab #3: [[Media:SEED_student.session4-PL.docx|docx]] [[Media:SEED_student.session4-PL.pdf|pdf]]
|  
|  
* Lab Day #3: Plasmid Mini-Prep [[Media:SEED_mini_prep.pdf|pdf]] [[Media:SEED_mini_prep.doc|doc]]
* Lab Day #3: Cloning and PCR  [[Media:SEED_session3.docx|docx]] [[Media:SEED_session3.pdf|pdf]]
* Restriction Digest [[Media:SEED_digest.pdf|pdf]] [[Media:SEED_digest.doc|doc]]
|-
|-
| [[../Day 4/]]
| [[../Day 4/]]
| Mar. 5
| Mar. 5
| PCR and gel
| miniprep, restriction digestion
|
|
* Homework #4:  
* Homework #4: [[Media:SEED_HW4-2011.docx|docx]] [[Media:SEED_HW4-2011.pdf|pdf]]
* Pre-Lab #4: [[Media:SEED_student.session5-PL.docx|docx]] [[Media:SEED_student.session5-PL.pdf|pdf]]
|  
|  
* Lab Day #4: PCR [[Media:SEED_PCR.pdf|pdf]] [[Media:SEED_PCR.doc|doc]]  
* Lab Day #4: Miniprep and Restriction Digestion  [[Media:SEED_session4.docx|docx]] [[Media:SEED_session4.pdf|pdf]]
* Gel Electrophoresis [[Media:SEED_gel_electrophoresis.pdf|pdf]] [[Media:SEED_gel_electrophoresis.doc|doc]]
|-
|-
| [[../Day 5/]]
| [[../Day 5/]]
| Mar. 12
| Mar. 12
| gel purification:
| gel electrophoresis
|
|
* Homework #5:  
* Homework #5: [[Media:SEED_HW5-2011.docx|docx]] [[Media:SEED_HW5-2011.pdf|pdf]]
| Lab Day #5: Gel Purification [[Media:SEED_gel_purification.pdf|pdf]] [[Media:SEED_gel_purification.doc|doc]]
* Pre-Lab #5: [[Media:SEED_student.session6-PL.docx|docx]] [[Media:SEED_student.session6-PL.pdf|pdf]]
* Quiz #2: [[Media:SEED_Quiz2.docx|docx]] [[Media:SEED_Quiz.pdf|pdf]]
|
* Lab Day #5: Gel Electrophoresis  [[Media:SEED_session5b.docx|docx]] [[Media:SEED_session5b.pdf|pdf]]
|-
|-
| [[../Day 6/]]
| [[../Day 6/]]
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| ligation, transformation
| ligation, transformation
|
|
* Pre-Lab #6:  
* Pre-Lab #6: none
| Lab Day #6: Ligation [[Media:SEED_ligation.pdf|pdf]] [[Media:SEED_ligation.doc|doc]]
* Midterm: [[Media:SEED_Midterm-2011.docx|docx]] [[Media:SEED_Midterm-2011.pdf|pdf]]
| Lab Day #6: Ligation and Transformation [[Media:SEED_session6.docx|docx]] [[Media:SEED_session6.pdf|pdf]]  
|-
|-
| [[../Day 7/]]
| [[../Day 7/]]
| Apr. 2
| Apr. 2
| prep for sequencing, glycerol  
| prep for sequencing, glycerol  
final project notes:
|
|
* Homework #7:  
* Homework #7: [[Media:SEED_HW7-2011.docx|docx]] [[Media:SEED_HW7-2011.pdf|pdf]]
* Pre-Lab #5:  
* Pre-Lab #6: [[Media:SEED_student.session8-PL.docx|docx]] [[Media:SEED_student.session8-PL.pdf|pdf]]
| Lab Day #7: Transformation [[Media:SEED_transformation.pdf|pdf]] [[Media:SEED_transformation.doc|doc]]
| Lab Day #7: [[Media:SEED_session7.docx|docx]] [[Media:SEED_session7.pdf|pdf]]
|-
|-
| [[../Day 8/]]
| [[../Day 8/]]
| Apr. 9
| Apr. 9
| β-gal assays, growth curves, viable cell titer, analyze sequencing
| analyze sequencing, smell test, final projects
|
|
* Homework #8:  
* Homework #8:  
| Lab Day #8: Beta-Galactosidase: Part I [[Media:SEED_beta_gal.pdf|pdf]] [[Media:SEED_beta_gal.doc|doc]]
| Lab Day #8:  
|-
|-
| [[../Day 9/]]
| [[../Day 9/]]
| Apr. 23
| Apr. 23
| device characterization and composability
| FINAL, final projects
|
|
* Homework #9: TBA
* Homework #9:  
| Lab Day #9: Beta-Galactosidase: Part II [[Media:SEED_inducibile_promoter.pdf|pdf]] [[Media:SEED_inducibile_promoter.doc|doc]]
| Lab Day #9:  
|-
|-
| [[../Day 10/]]
| [[../Day 10/]]
| April 30
| April 30
| data analysis, finish projects
| MIT 150!
|
|
* Final: TBA
*  
| Final Lab: pdf
|}
|}
</div>
</div>
</div>
</div>

Latest revision as of 03:58, 6 April 2011

[[../Overview/]] of the main lab project

Class Date Agenda Homework Lab Protocol
[[../Day 1/]] Feb. 5
  • Introductions
  • Molecular Biology Review
  • Units Review
  • Homework #1: doc pdf
    • read comic
    • iGEM Questions
    • Ideas (3-5) for what you want to engineer biology to do
    • Training lab questions
    • Data Analysis
 Lab Day #1: Serial Dilutions doc
[[../Day 2/]] Feb. 12 SynBio Overview, sterile technique, colony streaking, antibiotic resistance Lab Day #2: Plate Streaking pdf doc
[[../Day 3/]] Feb. 26 cloning, PCR
  • Lab Day #3: Cloning and PCR docx pdf
[[../Day 4/]] Mar. 5 miniprep, restriction digestion
  • Lab Day #4: Miniprep and Restriction Digestion docx pdf
[[../Day 5/]] Mar. 12 gel electrophoresis
  • Lab Day #5: Gel Electrophoresis docx pdf
[[../Day 6/]] Mar. 26 ligation, transformation Lab Day #6: Ligation and Transformation docx pdf
[[../Day 7/]] Apr. 2 prep for sequencing, glycerol Lab Day #7: docx pdf
[[../Day 8/]] Apr. 9 analyze sequencing, smell test, final projects
  • Homework #8:
 Lab Day #8:
[[../Day 9/]] Apr. 23 FINAL, final projects
  • Homework #9:
 Lab Day #9:
[[../Day 10/]] April 30 MIT 150!
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