SEED/2011/Schedule

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* Homework #2: [[Media:SEED_HW2-2011.docx|docx]] [[Media:SEED_HW2-2011.pdf|pdf]]
* Homework #2: [[Media:SEED_HW2-2011.docx|docx]] [[Media:SEED_HW2-2011.pdf|pdf]]
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* Pre-Lab: [[Media:SEED_student.session3-PL.docx|docx]] [[Media:SEED_student.session3-PL.pdf|pdf]]
| Lab Day #2: Plate Streaking [[Media:SEED_plate_streaking.pdf|pdf]] [[Media:SEED_plate_streaking.doc|doc]]  
| Lab Day #2: Plate Streaking [[Media:SEED_plate_streaking.pdf|pdf]] [[Media:SEED_plate_streaking.doc|doc]]  
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* Homework #3:  
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* Pre-Lab #3: [[Media:SEED_student.session3-PL.docx|docx]] [[Media:SEED_student.session3-PL.pdf|pdf]]
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* Pre-Lab #3:  
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* Lab Day #3: Cloning and PCR  [[Media:SEED_session3.docx|docx]] [[Media:SEED_session3.pdf|pdf]]   
* Lab Day #3: Cloning and PCR  [[Media:SEED_session3.docx|docx]] [[Media:SEED_session3.pdf|pdf]]   

Revision as of 08:42, 22 February 2011

Overview of the main lab project

Class Date Agenda Homework Lab Protocol
Day 1 Feb. 5
  • Introductions
  • Molecular Biology Review
  • Units Review
  • Homework #1: doc pdf
    • read comic
    • iGEM Questions
    • Ideas (3-5) for what you want to engineer biology to do
    • Training lab questions
    • Data Analysis
Lab Day #1: Serial Dilutions doc
Day 2 Feb. 12 SynBio Overview, sterile technique, colony streaking, antibiotic resistance Lab Day #2: Plate Streaking pdf doc
Day 3 Feb. 26 cloning, PCR
  • Homework #3:
  • Pre-Lab #3:
  • Lab Day #3: Cloning and PCR docx pdf
Day 4 Mar. 5 miniprep, restriction digestion
  • Homework #4:
  • Lab Day #4: Miniprep and Restriction Digestion
Day 5 Mar. 12 gel electrophoresis
  • Homework #5:
Lab Day #5: Gel Electrophoresis
Day 6 Mar. 26 ligation, transformation
  • Pre-Lab #6:
Lab Day #6: Ligation
Day 7 Apr. 2 prep for sequencing, glycerol

final project notes:

  • Homework #7:
  • Pre-Lab #5:
Lab Day #7:
Day 8 Apr. 9 β-gal assays, growth curves, viable cell titer, analyze sequencing
  • Homework #8:
Lab Day #8: Beta-Galactosidase: Part I
Day 9 Apr. 23 device characterization and composability
  • Homework #9:
Lab Day #9: Beta-Galactosidase: Part II
Day 10 April 30 data analysis, finish projects
  • Final:
Final Lab:
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