SEED/2011/Schedule
From OpenWetWare
(Difference between revisions)
| Line 58: | Line 58: | ||
* Homework #4: | * Homework #4: | ||
| | | | ||
| - | * Lab Day #4: Miniprep and Restriction Digestion | + | * Lab Day #4: Miniprep and Restriction Digestion [[Media:SEED_session4.docx|docx]] [[Media:SEED_session4.pdf|pdf]] |
|- | |- | ||
| [[../Day 5/]] | | [[../Day 5/]] | ||
Revision as of 10:17, 28 February 2011
Overview of the main lab project
| Class | Date | Agenda | Homework | Lab Protocol |
|---|---|---|---|---|
| Day 1 | Feb. 5 |
| Lab Day #1: Serial Dilutions doc | |
| Day 2 | Feb. 12 | SynBio Overview, sterile technique, colony streaking, antibiotic resistance | Lab Day #2: Plate Streaking pdf doc | |
| Day 3 | Feb. 26 | cloning, PCR | ||
| Day 4 | Mar. 5 | miniprep, restriction digestion |
| |
| Day 5 | Mar. 12 | gel electrophoresis |
| Lab Day #5: Gel Electrophoresis |
| Day 6 | Mar. 26 | ligation, transformation |
| Lab Day #6: Ligation |
| Day 7 | Apr. 2 | prep for sequencing, glycerol
final project notes: |
| Lab Day #7: |
| Day 8 | Apr. 9 | β-gal assays, growth curves, viable cell titer, analyze sequencing |
| Lab Day #8: Beta-Galactosidase: Part I |
| Day 9 | Apr. 23 | device characterization and composability |
| Lab Day #9: Beta-Galactosidase: Part II |
| Day 10 | April 30 | data analysis, finish projects |
| Final Lab: |
