SEED/2011/Schedule
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(Difference between revisions)
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| miniprep, restriction digestion | | miniprep, restriction digestion | ||
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| - | * Homework #4: | + | * Homework #4: [[Media:SEED_HW4-2011.docx|docx]] [[Media:SEED_HW4-2011.pdf|pdf]] |
| + | * Pre-Lab #4: [[Media:SEED_student.session5-PL.docx|docx]] [[Media:SEED_student.session5-PL.pdf|pdf]] | ||
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* Lab Day #4: Miniprep and Restriction Digestion [[Media:SEED_session4.docx|docx]] [[Media:SEED_session4.pdf|pdf]] | * Lab Day #4: Miniprep and Restriction Digestion [[Media:SEED_session4.docx|docx]] [[Media:SEED_session4.pdf|pdf]] | ||
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* Homework #5: | * Homework #5: | ||
| - | | Lab Day #5: Gel Electrophoresis | + | | Lab Day #5: Gel Electrophoresis [[Media:SEED_session5.docx|docx]] [[Media:SEED_session5.pdf|pdf]] |
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| [[../Day 6/]] | | [[../Day 6/]] | ||
Revision as of 21:19, 8 March 2011
Overview of the main lab project
| Class | Date | Agenda | Homework | Lab Protocol |
|---|---|---|---|---|
| Day 1 | Feb. 5 |
| Lab Day #1: Serial Dilutions doc | |
| Day 2 | Feb. 12 | SynBio Overview, sterile technique, colony streaking, antibiotic resistance | Lab Day #2: Plate Streaking pdf doc | |
| Day 3 | Feb. 26 | cloning, PCR | ||
| Day 4 | Mar. 5 | miniprep, restriction digestion | ||
| Day 5 | Mar. 12 | gel electrophoresis |
| Lab Day #5: Gel Electrophoresis docx pdf |
| Day 6 | Mar. 26 | ligation, transformation |
| Lab Day #6: Ligation |
| Day 7 | Apr. 2 | prep for sequencing, glycerol
final project notes: |
| Lab Day #7: |
| Day 8 | Apr. 9 | β-gal assays, growth curves, viable cell titer, analyze sequencing |
| Lab Day #8: Beta-Galactosidase: Part I |
| Day 9 | Apr. 23 | device characterization and composability |
| Lab Day #9: Beta-Galactosidase: Part II |
| Day 10 | April 30 | data analysis, finish projects |
| Final Lab: |
