SEED/2011/Schedule

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* Midterm: Midterm  [[Media:SEED_Midterm-2011.docx|docx]] [[Media:SEED_Midterm-2011.pdf|pdf]]
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* Midterm: [[Media:SEED_Midterm-2011.docx|docx]] [[Media:SEED_Midterm-2011.pdf|pdf]]
| Lab Day #6: Ligation and Transformation [[Media:SEED_session6.docx|docx]] [[Media:SEED_session6.pdf|pdf]]  
| Lab Day #6: Ligation and Transformation [[Media:SEED_session6.docx|docx]] [[Media:SEED_session6.pdf|pdf]]  
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Revision as of 19:01, 29 March 2011

Overview of the main lab project

Class Date Agenda Homework Lab Protocol
Day 1 Feb. 5
  • Introductions
  • Molecular Biology Review
  • Units Review
  • Homework #1: doc pdf
    • read comic
    • iGEM Questions
    • Ideas (3-5) for what you want to engineer biology to do
    • Training lab questions
    • Data Analysis
Lab Day #1: Serial Dilutions doc
Day 2 Feb. 12 SynBio Overview, sterile technique, colony streaking, antibiotic resistance Lab Day #2: Plate Streaking pdf doc
Day 3 Feb. 26 cloning, PCR
  • Lab Day #3: Cloning and PCR docx pdf
Day 4 Mar. 5 miniprep, restriction digestion
  • Lab Day #4: Miniprep and Restriction Digestion docx pdf
Day 5 Mar. 12 gel electrophoresis
  • Lab Day #5: Gel Electrophoresis docx pdf
Day 6 Mar. 26 ligation, transformation Lab Day #6: Ligation and Transformation docx pdf
Day 7 Apr. 2 prep for sequencing, glycerol

final project notes:

  • Homework #7:
  • Pre-Lab #5:
Lab Day #7:
Day 8 Apr. 9 β-gal assays, growth curves, viable cell titer, analyze sequencing
  • Homework #8:
Lab Day #8: Beta-Galactosidase: Part I
Day 9 Apr. 23 device characterization and composability
  • Homework #9:
Lab Day #9: Beta-Galactosidase: Part II
Day 10 April 30 data analysis, finish projects
  • Final:
Final Lab:
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