SEED/2011/Schedule

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[[../Overview/]] of the main lab project

Class Date Agenda Homework Lab Protocol
[[../Day 1/]] Feb. 5 safety training, training lab
  • Homework #1: doc pdf
    • read pdf
    • iGEM Questions
    • Ideas (3-5) for what you want to engineer biology to do
    • Training lab questions
    • Data Analysis
    • Pre-Lab
 Lab Day #1: Serial Dilutions pdf doc
[[../Day 2/]] Feb. 12 SynBio Overview, sterile technique, colony streaking, antibiotic resistance
  • Homework #2:
 Lab Day #2: Plate Streaking pdf doc
[[../Day 3/]] Feb. 26 miniprep, digest
  • Homework #3:
  • Pre-Lab #3:
  • Lab Day #3: Plasmid Mini-Prep pdf doc
  • Restriction Digest pdf doc
[[../Day 4/]] Mar. 5 PCR and gel
  • Homework #4:
[[../Day 5/]] Mar. 12 gel purification:
  • Homework #5:
 Lab Day #5: Gel Purification pdf doc
[[../Day 6/]] Mar. 26 ligation, transformation
  • Pre-Lab #6:
 Lab Day #6: Ligation pdf doc
[[../Day 7/]] Apr. 2 prep for sequencing, glycerol

final project notes:

  • Homework #7:
  • Pre-Lab #5:
 Lab Day #7: Transformation pdf doc
[[../Day 8/]] Apr. 9 β-gal assays, growth curves, viable cell titer, analyze sequencing
  • Homework #8:
 Lab Day #8: Beta-Galactosidase: Part I pdf doc
[[../Day 9/]] Apr. 23 device characterization and composability
  • Homework #9: TBA
 Lab Day #9: Beta-Galactosidase: Part II pdf doc
[[../Day 10/]] April 30 data analysis, finish projects
  • Final: TBA
 Final Lab: pdf
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