Class
|
Date
|
Lecture
|
Homework (due next week)
|
Lab
|
[[../Day 1/]]
|
Feb. 4
|
- Introductions
- Molecular Biology Review
- Units Review
- Course description & requirements
- Diagnostic test
|
- Homework #1:
- Pre-lab #1
- Post-lab #1
|
Lab Day #1: doc pdf
- Lab safety
- Concentrations & dilutions
- Using a micropipet
- Serial dilutions & spectrophotometer
|
[[../Day 2/]]
|
Feb. 11
|
- Goals of SynthBio
- What makes a good standardized part
- Why biology is hard to engineer
- Parts/devices/systems hierarchy and black-boxing
- Discuss Adventures in Synthbio and project ideas
|
- Homework #2: doc pdf
- Post-Lab #2
- Pre-Lab #3
|
Lab Day #2: doc pdf
- Overview of cloning process
- Minipreps
- Pre-Lab #2
|
No Class
|
Feb. 19
|
Presidents' Day
|
|
|
[[../Day 3/]]
|
Feb. 25
|
Polymerase chain reaction
|
|
Lab Day #3: doc pdf
- Polymerase Chain Reaction (PCR)
|
[[../Day 4/]]
|
Mar. 3
|
Restriction digest
|
- Homework #4: Three Ideas Report doc pdf
- (rough draft due Day 5, final draft due Day 6)
- Post-Lab #4
- Pre-Lab #5
|
Lab Day #4: doc pdf
|
[[../Day 5/]]
|
Mar. 10
|
Gel Electrophoresis & Purification
|
- Homework #5: Finish Three Ideas Report
- Post-Lab #5
- Pre-Lab #6
|
Lab Day #5: doc pdf
|
[[../Day 6/]]
|
Mar. 17
|
Ligation & transformation
|
- Homework #6: doc pdf
- Post-Lab #6
- Pre-Lab #7
|
Lab Day #6: doc pdf
- Ligation (morning)
- Transformation (afternoon)
|
[[../Day 7/]]
|
Mar. 24
|
- Sequencing to Verify Plasmids
- Preparing Freezer Stocks
|
- Homework #7: see [[../Day 8/]]
- Post-Lab #7
- Pre-Lab #8
|
Lab Day #7: doc pdf
- Posted lab packet does not include protocol, but does include all information necessary to do homework
- Miniprep for sequencing
- Prepare sequencing reaction to verify transformed strains
- Making glycerol freezer stocks of transformed strains
|
No SEED
|
Mar. 31
|
|
|
|
[[../Day 8/]]
|
Apr. 7
|
- Device diagrams
- Internal logic & timing of systems
|
- Homework #8: to be posted
- Post-Lab #8
- Pre-Lab #9
|
Lab Day #8: to be posted
- Analyzing sequencing results
- Measuring induction of fluorescent proteins
|
[[../Day 9/]]
|
Apr. 14
|
Projects!
|
- Homework #9: to be posted
- Post-lab #9
|
Lab Day #9: to be posted
- Measuring LacZ expression with various promoter/RBS combos
|
No SEED
|
Apr. 21
|
|
|
|
[[../Day 10/]]
|
April 28
|
- Designing a poster
- Presenting a poster
|
- Prepare to present projects!
|
Project work time
|
|
May 4
|
Final Presentations
|
- Kirsch Auditorium (room 32-123), Stata Center
- 6:30pm-8pm
|
|