SIL071906-Construction of pSB1A2-J23013

From OpenWetWare
Revision as of 13:17, 20 July 2006 by Bryanh (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

[TraGr]
PCR JL10/JL11 on Rlambda (?bp, EcoRI/SpeI, Neb2)
Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)
Product is pSB1A2-J23013

JL10 Forward EcoRI oligo to bioBrick TraG:
CCTAGGAATTCGCGGCCGCTTCTAGatgaagaaccgaaacaacgc
JL11 Reverse SpeI oligo to bioBrick TraG:
gacttACTAGTgaagcggctcatatcgtgatc

Retrieved from "https://openwetware.org/mediawiki/index.php?title=SIL071906-Construction_of_pSB1A2-J23013&oldid=52473"