SIL072006-Construction of pSB1A2-J23016: Difference between revisions
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F plasmid with trbC knockout | F plasmid with trbC knockout <br> | ||
PCR JL12/JL13 on pKD3 (~1000bp, dPN1, Neb2)<br> | PCR JL12/JL13 on pKD3 (~1000bp, dPN1, Neb2)<br> | ||
Electroporate into pKD46xpOX38<br> | Electroporate into pKD46xpOX38<br> | ||
Latest revision as of 11:22, 24 July 2006
F plasmid with trbC knockout
PCR JL12/JL13 on pKD3 (~1000bp, dPN1, Neb2)
Electroporate into pKD46xpOX38
Product is pSB1A2-J23016
JL12 Forward oligo to knockout trbC-f:
atgaagctgagtatgaaatctctggcagcactgctgatgatgctggtgCaggctggagctgcttcgaag
JL13 Reverse oligo to knockout trbC-f:
tcatttcccggaatctcctttccctgccagtaaatcatgagccactgggaaGtagccatggtccatat
