SIL072006-Construction of pSB1A2-J23017: Difference between revisions
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R plasmid with traG knockout | R plasmid with traG knockout | ||
PCR JL8/JL9 on pKD3 (~1000bp, dPN1, Neb2)<br> | |||
Electroporate into pKD46xRlambda<br> | |||
Product is pSB1A2-J23017 | |||
----------------------------- | |||
JL8 Forward oligo to knockout traG-r: <br> | |||
atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag<br> | |||
JL9 Reverse oligo to knockout traG-r: <br> | |||
tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg<br> | |||
Revision as of 13:29, 20 July 2006
R plasmid with traG knockout
PCR JL8/JL9 on pKD3 (~1000bp, dPN1, Neb2)
Electroporate into pKD46xRlambda
Product is pSB1A2-J23017
JL8 Forward oligo to knockout traG-r:
atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag
JL9 Reverse oligo to knockout traG-r:
tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg
