SIL072006-Construction of pSB1A2-J23017
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R plasmid with traG knockout
PCR JL8/JL9 on pKD3 (~1000bp, dPN1, Neb2)
Electroporate into pKD46xRlambda
Product is pSB1A2-J23017
JL8 Forward oligo to knockout traG-r:
atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag
JL9 Reverse oligo to knockout traG-r:
tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg
