SIL082506-Construction of pSB1AK3-J23074: Difference between revisions
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[RA2 Promoter][RBS][TraGr][TT] | [RA2 Promoter][RBS][TraGr][TT]<br> | ||
Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 ) | Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 )<br> | ||
Paste into pSB1AK3-J23026 (XbaI/BsaI, Neb2, Large 5229) | Paste into pSB1AK3-J23026 (XbaI/BsaI, Neb2, Large 5229)<br> | ||
Product is pSB1AK3-J23074. | Product is pSB1AK3-J23074. | ||
Revision as of 14:55, 25 August 2006
[RA2 Promoter][RBS][TraGr][TT]
Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 )
Paste into pSB1AK3-J23026 (XbaI/BsaI, Neb2, Large 5229)
Product is pSB1AK3-J23074.
