SIL082506-Construction of pSB1AK3-J23074: Difference between revisions
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<tt> | |||
[RA2 Promoter][RBS][TraGr][TT]<br> | [RA2 Promoter][RBS][TraGr][TT]<br> | ||
Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 )<br> | Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 )<br> | ||
Paste into pSB1AK3-J23026 (XbaI/BsaI, Neb2, Large 5229)<br> | Paste into pSB1AK3-J23026 (XbaI/BsaI, Neb2, Large 5229)<br> | ||
Product is pSB1AK3-J23074. | Product is pSB1AK3-J23074. | ||
[[User:JCAnderson|JCAnderson]] 20:11, 16 September 2006 (EDT)Revised construction: | |||
==Construction of pSB1A2-J23074== | |||
[Pcon][stRBS][traG][TT]<br> | |||
Digest pSB1AK3-J23026 . . . . . . . . (XbaI/PstI, small-2072 bp)<br> | |||
Digest pBca1020-J23038 . . . . . . . .(SpeI/PstI, large-2121 bp)<br> | |||
Product is pSB1A2-J23074<br> | |||
</tt> | |||
Latest revision as of 17:11, 16 September 2006
[RA2 Promoter][RBS][TraGr][TT]
Digest PCR product of pSB1A2-J23038 with G00101 and ca641R (BSAI/SpeI, Neb2, ~1600 )
Paste into pSB1AK3-J23026 (XbaI/BsaI, Neb2, Large 5229)
Product is pSB1AK3-J23074.
JCAnderson 20:11, 16 September 2006 (EDT)Revised construction:
Construction of pSB1A2-J23074
[Pcon][stRBS][traG][TT]
Digest pSB1AK3-J23026 . . . . . . . . (XbaI/PstI, small-2072 bp)
Digest pBca1020-J23038 . . . . . . . .(SpeI/PstI, large-2121 bp)
Product is pSB1A2-J23074
