SIL112706-Construction of pJ23006-J23201, 202, 203, 204, 205, 206, 207, 208: Difference between revisions
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J23201 SIL01 GTCATggatccataaaacccaaaagcaagaggtgattc | J23201 SIL01 GTCATggatccataaaacccaaaagcaagaggtgattc <br> | ||
SIL02 GATACaagctttttaactagaatcacctcttgcttttg | SIL02 GATACaagctttttaactagaatcacctcttgcttttg <br> | ||
J23202 SIL03 GTCATggatccataaaaaacccaaaagcaagaggtgattc | J23202 SIL03 GTCATggatccataaaaaacccaaaagcaagaggtgattc <br> | ||
SIL04 GATACaagctttttttaactagaatcacctcttgcttttg | SIL04 GATACaagctttttttaactagaatcacctcttgcttttg <br> | ||
J23203 SIL05 GTCATggatccataaaaaaaacccaaaagcaagaggtgattc | J23203 SIL05 GTCATggatccataaaaaaaacccaaaagcaagaggtgattc <br> | ||
SIL06 GATACaagctttttttttaactagaatcacctcttgcttttg | SIL06 GATACaagctttttttttaactagaatcacctcttgcttttg <br> | ||
J23204 SIL07 GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc | J23204 SIL07 GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc <br> | ||
SIL08 GATACaagctttttttttttttaactagaatcacctcttgcttttg | SIL08 GATACaagctttttttttttttaactagaatcacctcttgcttttg <br> | ||
J23205 SIL09 GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc | J23205 SIL09 GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc<br> | ||
SIL10 GATACaagctttttttttttttttaactagaatcacctcttgcttttg | SIL10 GATACaagctttttttttttttttaactagaatcacctcttgcttttg <br> | ||
J23206 SIL11 GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc | J23206 SIL11 GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br> | ||
SIL12 GATACaagctttttttttttttttttaactagaatcacctcttgcttttg | SIL12 GATACaagctttttttttttttttttaactagaatcacctcttgcttttg <br> | ||
J23207 SIL13 GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc | J23207 SIL13 GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br> | ||
SIL14 GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg | SIL14 GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg <br> | ||
J23208 SIL15 GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc | J23208 SIL15 GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br> | ||
SIL16 GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg | SIL16 GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg<br> | ||
Revision as of 15:12, 4 December 2006
Digest pJ23006-J23061 BamH/EcoRII Ligate with PCR product from SIL oligos
J23201 SIL01 GTCATggatccataaaacccaaaagcaagaggtgattc
SIL02 GATACaagctttttaactagaatcacctcttgcttttg
J23202 SIL03 GTCATggatccataaaaaacccaaaagcaagaggtgattc
SIL04 GATACaagctttttttaactagaatcacctcttgcttttg
J23203 SIL05 GTCATggatccataaaaaaaacccaaaagcaagaggtgattc
SIL06 GATACaagctttttttttaactagaatcacctcttgcttttg
J23204 SIL07 GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc
SIL08 GATACaagctttttttttttttaactagaatcacctcttgcttttg
J23205 SIL09 GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc
SIL10 GATACaagctttttttttttttttaactagaatcacctcttgcttttg
J23206 SIL11 GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc
SIL12 GATACaagctttttttttttttttttaactagaatcacctcttgcttttg
J23207 SIL13 GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc
SIL14 GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg
J23208 SIL15 GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc
SIL16 GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg
