SIL112706-Construction of pJ23006-J23201, 202, 203, 204, 205, 206, 207, 208: Difference between revisions

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Digest pJ23006-J23061 BamH/EcoRII
Digest pJ23006-J23061 BamH/EcoRII <br>
Ligate with PCR product from SIL oligos
Ligate with PCR product from SIL oligos


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J23201 SIL01 GTCATggatccataaaacccaaaagcaagaggtgattc <br>
*J23201 <br>
SIL02 GATACaagctttttaactagaatcacctcttgcttttg <br>
        SIL01 GTCATggatccataaaacccaaaagcaagaggtgattc <br>
        SIL02 GATACaagctttttaactagaatcacctcttgcttttg <br>
J23202 SIL03 GTCATggatccataaaaaacccaaaagcaagaggtgattc <br>
*J23202 <br>
SIL04 GATACaagctttttttaactagaatcacctcttgcttttg <br>
        SIL03 GTCATggatccataaaaaacccaaaagcaagaggtgattc <br>
        SIL04 GATACaagctttttttaactagaatcacctcttgcttttg <br>
J23203 SIL05 GTCATggatccataaaaaaaacccaaaagcaagaggtgattc <br>
*J23203 <br>
SIL06 GATACaagctttttttttaactagaatcacctcttgcttttg <br>
        SIL05 GTCATggatccataaaaaaaacccaaaagcaagaggtgattc <br>
        SIL06 GATACaagctttttttttaactagaatcacctcttgcttttg <br>
J23204 SIL07 GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc <br>
*J23204 <br>
SIL08 GATACaagctttttttttttttaactagaatcacctcttgcttttg <br>
        SIL07 GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc <br>
        SIL08 GATACaagctttttttttttttaactagaatcacctcttgcttttg <br>
J23205 SIL09 GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc<br>
*J23205 <br>
SIL10 GATACaagctttttttttttttttaactagaatcacctcttgcttttg <br>
        SIL09 GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc<br>
        SIL10 GATACaagctttttttttttttttaactagaatcacctcttgcttttg <br>
J23206 SIL11 GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br>
*J23206 <br>
SIL12 GATACaagctttttttttttttttttaactagaatcacctcttgcttttg <br>
        SIL11 GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br>
        SIL12 GATACaagctttttttttttttttttaactagaatcacctcttgcttttg <br>
J23207 SIL13 GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br>
*J23207 <br>
SIL14 GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg <br>
        SIL13 GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br>
        SIL14 GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg <br>
J23208 SIL15 GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br>
*J23208 <br>
SIL16 GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg<br>
        SIL15 GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br>
        SIL16 GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg<br>

Latest revision as of 15:15, 4 December 2006

Digest pJ23006-J23061 BamH/EcoRII
Ligate with PCR product from SIL oligos


  • J23201 
       SIL01	GTCATggatccataaaacccaaaagcaagaggtgattc		
					
       SIL02	GATACaagctttttaactagaatcacctcttgcttttg
  • J23202 
       SIL03	GTCATggatccataaaaaacccaaaagcaagaggtgattc	
					
       SIL04	GATACaagctttttttaactagaatcacctcttgcttttg
  • J23203 
       SIL05	GTCATggatccataaaaaaaacccaaaagcaagaggtgattc		
				
       SIL06	GATACaagctttttttttaactagaatcacctcttgcttttg
  • J23204 
       SIL07	GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc				
		
       SIL08	GATACaagctttttttttttttaactagaatcacctcttgcttttg
  • J23205 
       SIL09	GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc
			
       SIL10	GATACaagctttttttttttttttaactagaatcacctcttgcttttg
  • J23206 
       SIL11	GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc		
			
       SIL12	GATACaagctttttttttttttttttaactagaatcacctcttgcttttg
  • J23207 
       SIL13	GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc	
				
       SIL14	GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg
  • J23208 
       SIL15	GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc			
		
       SIL16	GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg
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