SIL112706-Construction of pJ23006-J23201, 202, 203, 204, 205, 206, 207, 208: Difference between revisions
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Digest pJ23006-J23061 BamH/EcoRII | Digest pJ23006-J23061 BamH/EcoRII <br> | ||
Ligate with PCR product from SIL oligos | Ligate with PCR product from SIL oligos | ||
| Line 6: | Line 6: | ||
---- | ---- | ||
J23201 SIL01 GTCATggatccataaaacccaaaagcaagaggtgattc <br> | *J23201 <br> | ||
SIL01 GTCATggatccataaaacccaaaagcaagaggtgattc <br> | |||
SIL02 GATACaagctttttaactagaatcacctcttgcttttg <br> | SIL02 GATACaagctttttaactagaatcacctcttgcttttg <br> | ||
J23202 SIL03 GTCATggatccataaaaaacccaaaagcaagaggtgattc <br> | *J23202 <br> | ||
SIL03 GTCATggatccataaaaaacccaaaagcaagaggtgattc <br> | |||
SIL04 GATACaagctttttttaactagaatcacctcttgcttttg <br> | SIL04 GATACaagctttttttaactagaatcacctcttgcttttg <br> | ||
J23203 SIL05 GTCATggatccataaaaaaaacccaaaagcaagaggtgattc <br> | *J23203 <br> | ||
SIL05 GTCATggatccataaaaaaaacccaaaagcaagaggtgattc <br> | |||
SIL06 GATACaagctttttttttaactagaatcacctcttgcttttg <br> | SIL06 GATACaagctttttttttaactagaatcacctcttgcttttg <br> | ||
J23204 SIL07 GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc <br> | *J23204 <br> | ||
SIL07 GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc <br> | |||
SIL08 GATACaagctttttttttttttaactagaatcacctcttgcttttg <br> | SIL08 GATACaagctttttttttttttaactagaatcacctcttgcttttg <br> | ||
J23205 SIL09 GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc<br> | *J23205 <br> | ||
SIL09 GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc<br> | |||
SIL10 GATACaagctttttttttttttttaactagaatcacctcttgcttttg <br> | SIL10 GATACaagctttttttttttttttaactagaatcacctcttgcttttg <br> | ||
J23206 SIL11 GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br> | *J23206 <br> | ||
SIL11 GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br> | |||
SIL12 GATACaagctttttttttttttttttaactagaatcacctcttgcttttg <br> | SIL12 GATACaagctttttttttttttttttaactagaatcacctcttgcttttg <br> | ||
J23207 SIL13 GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br> | *J23207 <br> | ||
SIL13 GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br> | |||
SIL14 GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg <br> | SIL14 GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg <br> | ||
J23208 SIL15 GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br> | *J23208 <br> | ||
SIL15 GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc <br> | |||
SIL16 GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg<br> | SIL16 GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg<br> | ||
Revision as of 15:14, 4 December 2006
Digest pJ23006-J23061 BamH/EcoRII
Ligate with PCR product from SIL oligos
- J23201
SIL01 GTCATggatccataaaacccaaaagcaagaggtgattc
SIL02 GATACaagctttttaactagaatcacctcttgcttttg
- J23202
SIL03 GTCATggatccataaaaaacccaaaagcaagaggtgattc
SIL04 GATACaagctttttttaactagaatcacctcttgcttttg
- J23203
SIL05 GTCATggatccataaaaaaaacccaaaagcaagaggtgattc
SIL06 GATACaagctttttttttaactagaatcacctcttgcttttg
- J23204
SIL07 GTCATggatccataaaaaaaaaaaacccaaaagcaagaggtgattc
SIL08 GATACaagctttttttttttttaactagaatcacctcttgcttttg
- J23205
SIL09 GTCATggatccataaaaaaaaaaaaaacccaaaagcaagaggtgattc
SIL10 GATACaagctttttttttttttttaactagaatcacctcttgcttttg
- J23206
SIL11 GTCATggatccataaaaaaaaaaaaaaaacccaaaagcaagaggtgattc
SIL12 GATACaagctttttttttttttttttaactagaatcacctcttgcttttg
- J23207
SIL13 GTCATggatccataaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc
SIL14 GATACaagctttttttttttttttttttaactagaatcacctcttgcttttg
- J23208
SIL15 GTCATggatccataaaaaaaaaaaaaaaaaaaacccaaaagcaagaggtgattc
SIL16 GATACaagctttttttttttttttttttttaactagaatcacctcttgcttttg
