SOB
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(Difference between revisions)
| Line 7: | Line 7: | ||
*10 mM NaCl | *10 mM NaCl | ||
*2.5 mM KCl | *2.5 mM KCl | ||
| - | |||
*20 mM MgSO<sub>4</sub> | *20 mM MgSO<sub>4</sub> | ||
| + | |||
| + | Per liter: | ||
| + | *5 g yeast extract | ||
| + | *20 g tryptone | ||
| + | *0.584 g NaCl | ||
| + | *0.186 g KCl | ||
| + | *2.4 g MgSO<sub>4</sub> | ||
| + | |||
| + | ''Note:'' Some formulations of SOB also include 10 mM MgCl<sub>2</sub>. | ||
==Source== | ==Source== | ||
| Line 14: | Line 22: | ||
F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36 | F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36 | ||
| + | |||
| + | ==Trivia== | ||
| + | SOB supposedly stands for super optimal broth. | ||
Revision as of 13:45, 15 April 2006
Contents |
Summary
SOB Medium. Used in growing bacteria for preparing chemically competent cells
Ingredients
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter:
- 5 g yeast extract
- 20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 2.4 g MgSO4
Note: Some formulations of SOB also include 10 mM MgCl2.
Source
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
Trivia
SOB supposedly stands for super optimal broth.
