SOB: Difference between revisions
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==15/10 medium== | ==15/10 medium== | ||
Growth of competent TOP10 cells in the | Growth of competent TOP10 cells in Example 2 of the Bloom05 patent is performed in 15/10 broth, which is similar to SOB: | ||
* 1.5% yeast extract | * 1.5% yeast extract | ||
| Line 31: | Line 31: | ||
* 10 mM MgCl<sub>2</sub> | * 10 mM MgCl<sub>2</sub> | ||
* 10 mM MgSO<sub>4</sub> | * 10 mM MgSO<sub>4</sub> | ||
==Source== | ==Source== | ||
Revision as of 14:04, 9 May 2006
Summary
SOB Medium. Used in growing bacteria for preparing chemically competent cells
Ingredients
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 20 mM MgSO4
Per liter:
- 5 g yeast extract
- 20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 2.4 g MgSO4
Note: Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.
SOB medium is also available dry premixed from Difco, 0443-17.
Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.
15/10 medium
Growth of competent TOP10 cells in Example 2 of the Bloom05 patent is performed in 15/10 broth, which is similar to SOB:
- 1.5% yeast extract
- 1% Bacto-Tryptone
- 10mM NaCl
- 2mM KCl
- 10 mM MgCl2
- 10 mM MgSO4
Source
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
Trivia
SOB supposedly stands for super optimal broth.
