SOC

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(added tags)
m (Protocol)
Line 9: Line 9:
#Follow directions to make 1 liter of [[SOB]] media
#Follow directions to make 1 liter of [[SOB]] media
#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
 +
 +
*2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
 +
 +
{| border="1" cellpadding="5" cellspacing="0" align="left"
 +
|+
 +
! Reagent
 +
! for 1 L
 +
! 500 mL
 +
! 100 mL
 +
|-=
 +
| 2 M glucose
 +
|10 ml
 +
| 5 ml
 +
| 1 ml
 +
|}
 +
*aliquote in 2 ml tubes
 +
*store at -20°C
==Source==
==Source==

Revision as of 23:05, 29 December 2011

Contents

Summary

SOC Medium. Used in Bacterial Transformation.

Ingredients

  • SOB
  • 20 mM glucose

Protocol

  1. Follow directions to make 1 liter of SOB media
  2. After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
  • 2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
Reagent for 1 L 500 mL 100 mL
2 M glucose 10 ml 5 ml 1 ml
  • aliquote in 2 ml tubes
  • store at -20°C

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

The "C" in SOC supposedly stands for catabolite repression due to the added glucose.

Personal tools