SOC: Difference between revisions
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#Follow directions to make 1 liter of [[SOB]] media | #Follow directions to make 1 liter of [[SOB]] media | ||
#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution | #After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution | ||
*2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube | |||
{| border="1" cellpadding="5" cellspacing="0" align="left" | |||
|+ | |||
! Reagent | |||
! for 1 L | |||
! 500 mL | |||
! 100 mL | |||
|-= | |||
| 2 M glucose | |||
|10 ml | |||
| 5 ml | |||
| 1 ml | |||
|} | |||
*aliquote in 2 ml tubes | |||
*store at -20°C | |||
==Source== | ==Source== | ||
Revision as of 20:05, 29 December 2011
Summary
SOC Medium. Used in Bacterial Transformation.
Ingredients
- SOB
- 20 mM glucose
Protocol
- Follow directions to make 1 liter of SOB media
- After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
- 2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
| Reagent | for 1 L | 500 mL | 100 mL |
|---|---|---|---|
| 2 M glucose | 10 ml | 5 ml | 1 ml |
- aliquote in 2 ml tubes
- store at -20°C
Source
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
Trivia
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.
