SOC

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== Summary ==
== Summary ==
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SOC Medium.  Used in bacterial transformations
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SOC Medium.  Used in [[Bacterial Transformation]].
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== Ingredients ==
== Ingredients ==
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*0.5% (w/v) yeast extract
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*[[SOB]]
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*2% (w/v) tryptone
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*10 mM NaCl
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*2.5 mM KCl
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*10 mM MgCl<sub>2</sub>
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*20 mM MgSO<sub>4</sub>
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*20 mM glucose
*20 mM glucose
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==Protocol==
==Protocol==
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#SOB (SOC - glucose) is available at the kitchen
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#Follow directions to make 1 liter of [[SOB]] media
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#Add 1/100 volume 40% glucose (also available from the kitchen)
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#After cooling medium to less than 50&deg;C, add 20 ml filter sterilized 20% glucose solution
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==Alternative==
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#2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
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#aliquote in 2 ml tubes
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#store at -20°C
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{| border="1" cellpadding="5" cellspacing="0"
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|+
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! Reagent
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! for 1 L
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! 500 mL
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! 100 mL
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|-=
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| 2 M glucose  
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|10 ml
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| 5 ml
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| 1 ml
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|}
==Source==
==Source==
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F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36
F. Ausubel et al., ''Short Protocols in Molecular Biology'' (John Wiley & Sons, ed. 4, 1999) pg. A1-36
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==Trivia==
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The "C" in SOC supposedly stands for catabolite repression due to the added glucose.
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[[Category:Material]]  [[Category:Standard Media]]

Current revision

Contents

Summary

SOC Medium. Used in Bacterial Transformation.

Ingredients

  • SOB
  • 20 mM glucose

Protocol

  1. Follow directions to make 1 liter of SOB media
  2. After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution


Alternative

  1. 2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
  2. aliquote in 2 ml tubes
  3. store at -20°C
Reagent for 1 L 500 mL 100 mL
2 M glucose 10 ml 5 ml 1 ml

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

The "C" in SOC supposedly stands for catabolite repression due to the added glucose.

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