SOC
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#Follow directions to make 1 liter of [[SOB]] media | #Follow directions to make 1 liter of [[SOB]] media | ||
#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution | #After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution | ||
| + | |||
| + | |||
| + | ==Alternative== | ||
| + | #2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube | ||
| + | #aliquote in 2 ml tubes | ||
| + | #store at -20°C | ||
| + | {| border="1" cellpadding="5" cellspacing="0" | ||
| + | |+ | ||
| + | ! Reagent | ||
| + | ! for 1 L | ||
| + | ! 500 mL | ||
| + | ! 100 mL | ||
| + | |-= | ||
| + | | 2 M glucose | ||
| + | |10 ml | ||
| + | | 5 ml | ||
| + | | 1 ml | ||
| + | |} | ||
==Source== | ==Source== | ||
Current revision
Contents |
Summary
SOC Medium. Used in Bacterial Transformation.
Ingredients
- SOB
- 20 mM glucose
Protocol
- Follow directions to make 1 liter of SOB media
- After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
Alternative
- 2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
- aliquote in 2 ml tubes
- store at -20°C
| Reagent | for 1 L | 500 mL | 100 mL |
|---|---|---|---|
| 2 M glucose | 10 ml | 5 ml | 1 ml |
Source
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
Trivia
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.
