SOC

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (Protocol)
Current revision (17:33, 25 January 2013) (view source)
(fixed heading)
 
(3 intermediate revisions not shown.)
Line 10: Line 10:
#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution
-
*2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
+
 
-
+
==Alternative==
-
{| border="1" cellpadding="5" cellspacing="0" align="left"  
+
#2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
 +
#aliquote in 2 ml tubes
 +
#store at -20°C
 +
{| border="1" cellpadding="5" cellspacing="0"
|+  
|+  
! Reagent
! Reagent
Line 24: Line 27:
| 1 ml
| 1 ml
|}
|}
-
*aliquote in 2 ml tubes
 
-
*store at -20°C
 
==Source==
==Source==

Current revision

Contents

Summary

SOC Medium. Used in Bacterial Transformation.

Ingredients

  • SOB
  • 20 mM glucose

Protocol

  1. Follow directions to make 1 liter of SOB media
  2. After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution


Alternative

  1. 2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
  2. aliquote in 2 ml tubes
  3. store at -20°C
Reagent for 1 L 500 mL 100 mL
2 M glucose 10 ml 5 ml 1 ml

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

The "C" in SOC supposedly stands for catabolite repression due to the added glucose.

Personal tools