SOC

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (Protocol)
Current revision (18:33, 25 January 2013) (view source)
(fixed heading)
 
Line 11: Line 11:
-
===Alternative==
+
==Alternative==
#2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
#2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
#aliquote in 2 ml tubes
#aliquote in 2 ml tubes

Current revision

Contents

Summary

SOC Medium. Used in Bacterial Transformation.

Ingredients

  • SOB
  • 20 mM glucose

Protocol

  1. Follow directions to make 1 liter of SOB media
  2. After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution


Alternative

  1. 2M glucose stock: dissolve 18 g glucose into 50 ml (final volume) ddH2O and filter-sterilize into sterile 50 ml tube
  2. aliquote in 2 ml tubes
  3. store at -20°C
Reagent for 1 L 500 mL 100 mL
2 M glucose 10 ml 5 ml 1 ml

Source

Adapted From:

F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36

Trivia

The "C" in SOC supposedly stands for catabolite repression due to the added glucose.

Personal tools