SOC
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Summary
SOC Medium. Used in bacterial transformations
Ingredients
- 0.5% (w/v) yeast extract
- 2% (w/v) tryptone
- 10 mM NaCl
- 2.5 mM KCl
- 10 mM MgCl2
- 20 mM MgSO4
- 20 mM glucose
Protocol
- SOB (SOC - glucose) is available at the kitchen
- Add 1/100 volume 40% glucose (also available from the kitchen)
Source
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36