SSC
From OpenWetWare
(Difference between revisions)
(20x recipe) |
(lead section) |
||
| Line 1: | Line 1: | ||
| - | SSC buffer | + | '''SSC buffer''' is named after its ingredients '''s'''aline (sodium chloride) and '''s'''odium '''c'''itrate. It is used as a buffer for blotting, hybridisation, and stringency washes in [[Northern]] and [[Southern blot]]s as well as DNA microarray. |
== 1x SSC buffer == | == 1x SSC buffer == | ||
| Line 13: | Line 13: | ||
* sterilize by autoclaving | * sterilize by autoclaving | ||
| - | === using stock | + | === using stock solutions === |
* 3M NaCl | * 3M NaCl | ||
* 300 mM sodium citrate, pH 7.0 | * 300 mM sodium citrate, pH 7.0 | ||
| + | |||
| + | == See also == | ||
| + | * [http://en.wikipedia.org/wiki/SSC_buffer Wikipedia: SSC buffer] | ||
[[Category:Material]] and [[Category:Buffers]] | [[Category:Material]] and [[Category:Buffers]] | ||
Revision as of 08:38, 15 June 2009
SSC buffer is named after its ingredients saline (sodium chloride) and sodium citrate. It is used as a buffer for blotting, hybridisation, and stringency washes in Northern and Southern blots as well as DNA microarray.
Contents |
1x SSC buffer
- 150mM NaCl
- 15mM sodium citrate
20x SSC
- 800ml dH2O
- 175.3g NaCl (3M)
- 88.2g trisodium citrate (NaCit; 300mM; e.g. S4641)
- adjust the pH to 7.0 with a few drops of 1M HCl
- adjust the volume to 1L with dH2O
- sterilize by autoclaving
using stock solutions
- 3M NaCl
- 300 mM sodium citrate, pH 7.0
