STEM Facility: Difference between revisions

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'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  


at Brookhaven National Laboratory
        in the Biology Deprtment at Brookhaven National Laboratory


The STEM is an NIH Research Resource and as such is available to outside users with appropriate projects free of charge.
The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.


See our web page  http://www.biology.bnl.gov/stem/stem.html for details.
After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis.  We had twenty-five outside users (outside BNL) this year (2008).  It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition. 
 
The microscope is now running with our new computer system.  A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.
 
Our web page is being revised.  (see it (reload or refresh) http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions.  A brief summary of the Fee-for-Service is below:
 
 
'''FEE-FOR-SERVICE'''
 
Details on our web page include:
*Services Provided
*Guarantee
 
SAMPLE CHARGE 
 
Biology’s Scanning Transmission Electron Microscope (STEM)
 
Per Grid* – non-proprietary…………………………..$391.02**
 
Minimum of 3 grids per specimen




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'''FIRST NEW POSTING'''
'''USERS'''
 
Potential users (from January on) should contact either Joe or Beth.  Probably e-mail is better than the telephone.  Joe and Beth will keep the User Program running, on a half-time basis. 


STEM Users who use the STEM PCMass programs for analyses and viewing, should be using a relatively recent version, say PCMass28 or some PCMass29.  The newer versions are much more user-friendly and also have more tutorials.


There should be a new, complete, PCMass29 soon.
'''NEWS'''


Martha Simon and Frank Kito retired January 2, 2009.  Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.


'''GOLD-LABELING WORKSHOP'''


'''ANNUAL EM WORKSHOP'''


                          Gold Labeling Workshop
No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.
Sept. 11-13, 2007
Sponsored by the Brookhaven National Lab STEM (scanning transmission EM) Facility
A Hands-on Tutorial that will cover both theory and practice.  Topics will include:
Gold nanoparticles:  structure, synthesis, and properties
Molecular labeling strategies (to amines, thiols, genetic tags, active sites, complexes, nucleic acids, peptides)
Ni-NTA-gold
Sizes of gold from 0.8 nm to 30 nm
Isolation of labeled products (various chromatographies and other methods)
Unstained, negatively stained and Cryo samples
Comparison of STEM and TEM
Silver and gold enhancement
Image processing using gold labels
Problems and how to overcome them (e.g., labeling yield/occupancy, non-specific binding)
Other uses of gold nanoparticles (Western blots, light microscopy, medicine)
Laboratory participation to gold label a protein for EM
What’s new

Latest revision as of 13:59, 29 July 2009

THE STEM (Scanning Transmission Electron Microscope) FACILITY

       in the Biology Deprtment at Brookhaven National Laboratory

The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.

After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis. We had twenty-five outside users (outside BNL) this year (2008). It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.

The microscope is now running with our new computer system. A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.

Our web page is being revised. (see it (reload or refresh) http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions. A brief summary of the Fee-for-Service is below:


FEE-FOR-SERVICE

Details on our web page include:

  • Services Provided
  • Guarantee

SAMPLE CHARGE

Biology’s Scanning Transmission Electron Microscope (STEM)

Per Grid* – non-proprietary…………………………..$391.02**

Minimum of 3 grids per specimen


PURPOSE OF THIS PAGE

Our web page (link given above) has essentially all the useful information about the facility and its use.

We will try to use this page for transient information, such as announcements and "news".


USERS

Potential users (from January on) should contact either Joe or Beth. Probably e-mail is better than the telephone. Joe and Beth will keep the User Program running, on a half-time basis.


NEWS

Martha Simon and Frank Kito retired January 2, 2009. Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.


ANNUAL EM WORKSHOP

No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.