STEM Facility

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'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
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at Brookhaven National Laboratory
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        in the Biology Deprtment at Brookhaven National Laboratory
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The STEM is an NIH Research Resource and as such is available to outside users with appropriate projects free of charge.
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The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.
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See our web page http://www.biology.bnl.gov/stem/stem.html for details.
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After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis. We had twenty-five outside users (outside BNL) this year (2008). It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.
 +
The microscope is now running with our new computer system.  A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.
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'''PURPOSE OF THIS PAGE'''
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Our web page is being revised.  (see it (reload or refresh)  http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions.  A brief summary of the Fee-for-Service is below:
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Our web page (link given above) has essentially all the useful information about the facility and its use.
 
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We will try to use this page for transient information, such as announcements and "news".
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'''FEE-FOR-SERVICE'''
 +
Details on our web page include:
 +
*Services Provided
 +
*Guarantee
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'''A NEW POSTING'''
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SAMPLE CHARGE 
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STEM Users who use the STEM PCMass programs for analyses and viewing, should be using a relatively recent version, say PCMass28 or some PCMass29.  The newer versions are much more user-friendly and also have more tutorials.
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Biology’s Scanning Transmission Electron Microscope (STEM)
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There should be a new, complete, PCMass29 soon.
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Per Grid* – non-proprietary…………………………..$391.02**
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Minimum of 3 grids per specimen
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'''COURSE ANNOUNCEMENT'''
 
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*'''GOLD-LABELING WORKSHOP'''
 
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Sept. 11-13, 2007
 
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Sponsored by the Brookhaven National Lab STEM (scanning transmission EM) Facility
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'''PURPOSE OF THIS PAGE'''
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A Hands-on Tutorial that will cover both theory and practice.
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Our web page (link given above) has essentially all the useful information about the facility and its use.
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Topics will include:
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We will try to use this page for transient information, such as announcements and "news".
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*Gold nanoparticles:  structure, synthesis, and properties
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*Molecular labeling strategies (to amines, thiols, genetic tags, active sites, complexes, nucleic acids, peptides)
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*Ni-NTA-gold
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*Sizes of gold from 0.8 nm to 30 nm
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*Isolation of labeled products (various chromatographies and other methods)
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*Unstained, negatively stained and Cryo samples
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*Comparison of STEM and TEM
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*Silver and gold enhancement
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*Image processing using gold labels
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*Problems and how to overcome them (e.g., labeling yield/occupancy, non-specific binding)
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*Other uses of gold nanoparticles (Western blots, light microscopy, medicine)
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*Laboratory participation to gold label a protein for EM
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*What’s new
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Limited Enrollment (12) due to lab space: 
 
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*A course fee of $650 covers housing, meals (dorm, cafeteria), and materials.
 
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Proposals to attend will be refereed:
 
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*submit CV and letter describing you and your lab’s labeling interest.
 
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Optionally describe a sample of yours that, if selected, would be labeled during the course.
 
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Submit registration request by July 1 to:
 
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*Dr. James Hainfeld by email: hainfeld@bnl.gov
 
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Brookhaven National Laboratory, Biology Dept., Bldg. 463, Upton, NY  11973, USA
 
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Tel.  631-344-3372  *  Fax. 631-344-3407
 
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http://www.biology.bnl.gov/stem/stem.html
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'''USERS'''
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Potential users (from January on) should contact either Joe or Beth.  Probably e-mail is better than the telephone.  Joe and Beth will keep the User Program running, on a half-time basis. 
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 +
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'''NEWS'''
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Martha Simon and Frank Kito retired January 2, 2009.  Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.
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'''USERS'''
 
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Most users mail (ON Express) their samples.  Frozen samples, in a well-insulated package with lots of dry ice, will stay frozen for many days -- long enough to clear customs if they are from abroad.  A sample package is shown below (held by Joe and Beth):
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'''ANNUAL EM WORKSHOP'''
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Retrieved from "http://www.openwetware.org/wiki/Image:--Package-file.jpg--"
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No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.

Current revision

THE STEM (Scanning Transmission Electron Microscope) FACILITY

       in the Biology Deprtment at Brookhaven National Laboratory

The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.

After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis. We had twenty-five outside users (outside BNL) this year (2008). It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.

The microscope is now running with our new computer system. A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.

Our web page is being revised. (see it (reload or refresh) http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions. A brief summary of the Fee-for-Service is below:


FEE-FOR-SERVICE

Details on our web page include:

  • Services Provided
  • Guarantee

SAMPLE CHARGE

Biology’s Scanning Transmission Electron Microscope (STEM)

Per Grid* – non-proprietary…………………………..$391.02**

Minimum of 3 grids per specimen


PURPOSE OF THIS PAGE

Our web page (link given above) has essentially all the useful information about the facility and its use.

We will try to use this page for transient information, such as announcements and "news".


USERS

Potential users (from January on) should contact either Joe or Beth. Probably e-mail is better than the telephone. Joe and Beth will keep the User Program running, on a half-time basis.


NEWS

Martha Simon and Frank Kito retired January 2, 2009. Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.


ANNUAL EM WORKSHOP

No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.

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