STEM Facility

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'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
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at Brookhaven National Laboratory
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        in the Biology Deprtment at Brookhaven National Laboratory
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The STEM is an NIH Research Resource and as such is available to outside users with appropriate projects free of charge.
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The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.
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See our web page http://www.biology.bnl.gov/stem/stem.html for details.
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After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis. We had twenty-five outside users (outside BNL) this year (2008). It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.
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The microscope is now running with our new computer system.  A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.
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'''PURPOSE OF THIS PAGE'''
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Our web page is being revised.  (see it (reload or refresh)  http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions.  A brief summary of the Fee-for-Service is below:
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Our web page (link given above) has essentially all the useful information about the facility and its use.
 
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We will try to use this page for transient information, such as announcements and "news".
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'''FEE-FOR-SERVICE'''
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Details on our web page include:
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*Services Provided
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*Guarantee
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'''A NEW POSTING'''
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SAMPLE CHARGE 
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STEM Users who use the STEM PCMass programs for analyses and viewing, should be using a relatively recent version, say PCMass28 or some PCMass29.  The newer versions are much more user-friendly and also have more tutorials.
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Biology’s Scanning Transmission Electron Microscope (STEM)
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There should be a new, complete, PCMass29 soon.
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Per Grid* – non-proprietary…………………………..$391.02**
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Minimum of 3 grids per specimen
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'''A GOLD-LABELING WORKSHOP'''
 
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Was held Sept. 11-13, 2007
 
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Organized by Dr. James Hainfeld
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'''PURPOSE OF THIS PAGE'''
 +
 
 +
Our web page (link given above) has essentially all the useful information about the facility and its use.
 +
 
 +
We will try to use this page for transient information, such as announcements and "news".
'''USERS'''
'''USERS'''
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Most users mail (ON Express) their samplesFrozen samples, in a well-insulated package with lots of dry ice, will stay frozen for many days -- long enough to clear customs if they are from abroadA sample package is shown below (held by Joe and Beth):
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Potential users (from January on) should contact either Joe or BethProbably e-mail is better than the telephone.  Joe and Beth will keep the User Program running, on a half-time basis.   
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Retrieved from "http://www.openwetware.org/wiki/Image:--Package-file.jpg--"
 
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'''NEWS'''
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'''ANNUAL EM WORKSHOP'''
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Martha Simon and Frank Kito retired January 2, 2009.  Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.
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The annual STEM/EM Workshop was held Friday, April 27th, at the end of the RapiData Crystallography course.  Here is a picture of the participants.
 
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[[Image:EMWorkshopRapiData2007.jpg]]
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'''ANNUAL EM WORKSHOP'''
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No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.

Current revision

THE STEM (Scanning Transmission Electron Microscope) FACILITY

       in the Biology Deprtment at Brookhaven National Laboratory

The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.

After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis. We had twenty-five outside users (outside BNL) this year (2008). It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.

The microscope is now running with our new computer system. A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.

Our web page is being revised. (see it (reload or refresh) http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions. A brief summary of the Fee-for-Service is below:


FEE-FOR-SERVICE

Details on our web page include:

  • Services Provided
  • Guarantee

SAMPLE CHARGE

Biology’s Scanning Transmission Electron Microscope (STEM)

Per Grid* – non-proprietary…………………………..$391.02**

Minimum of 3 grids per specimen


PURPOSE OF THIS PAGE

Our web page (link given above) has essentially all the useful information about the facility and its use.

We will try to use this page for transient information, such as announcements and "news".


USERS

Potential users (from January on) should contact either Joe or Beth. Probably e-mail is better than the telephone. Joe and Beth will keep the User Program running, on a half-time basis.


NEWS

Martha Simon and Frank Kito retired January 2, 2009. Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.


ANNUAL EM WORKSHOP

No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.

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