STEM Facility: Difference between revisions

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'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  


at Brookhaven National Laboratory
        in the Biology Deprtment at Brookhaven National Laboratory


The STEM used to be an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.
The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.
We have lost this support, but continue to operate as a User Facility on a Fee-For-Service basis.


We are updating our web page.
After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis.  We had twenty-five outside users (outside BNL) this year (2008).  It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.


See it (reload or refresh)  http://www.biology.bnl.gov/stem/stem.html for details.
The microscope is now running with our new computer system.  A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.


Our web page is being revised.  (see it (reload or refresh)  http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions.  A brief summary of the Fee-for-Service is below:


'''PURPOSE OF THIS PAGE'''


Our web page (link given above) has essentially all the useful information about the facility and its use.
'''FEE-FOR-SERVICE'''
 
Details on our web page include:
*Services Provided
*Guarantee


We will try to use this page for transient information, such as announcements and "news".
SAMPLE CHARGE 


Biology’s Scanning Transmission Electron Microscope (STEM)


'''A NEW POSTING'''
Per Grid* – non-proprietary…………………………..$391.02**


STEM Users who use the STEM PCMass programs for analyses and viewing, should be using a relatively recent version, say PCMass28 or some PCMass29.  The newer versions are much more user-friendly and also have more tutorials.
Minimum of 3 grids per specimen


There should be a new, complete, PCMass29 soon.


'''PURPOSE OF THIS PAGE'''


'''A GOLD-LABELING WORKSHOP'''
Our web page (link given above) has essentially all the useful information about the facility and its use.
                         
Was held Sept. 11-13, 2007


Organized by Dr. James Hainfeld
We will try to use this page for transient information, such as announcements and "news".




'''USERS'''
'''USERS'''


Most users mail (ON Express) their samples.  Frozen samples, in a well-insulated package with lots of dry ice, will stay frozen for many days -- long enough to clear customs if they are from abroad.  A sample package is shown below (held by Joe and Beth):
Potential users (from January on) should contact either Joe or Beth.  Probably e-mail is better than the telephone.  Joe and Beth will keep the User Program running, on a half-time basis.   


Retrieved from "http://www.openwetware.org/wiki/Image:--Package-file.jpg--"
 
'''NEWS'''
 
Martha Simon and Frank Kito retired January 2, 2009.   Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.




'''ANNUAL EM WORKSHOP'''
'''ANNUAL EM WORKSHOP'''


The annual STEM/EM Workshop was held Friday, April 27th, at the end of the RapiData Crystallography course. Here is a picture of the participants.
No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.
 
[[Image:EMWorkshopRapiData2007.jpg]]

Latest revision as of 13:59, 29 July 2009

THE STEM (Scanning Transmission Electron Microscope) FACILITY 

       in the Biology Deprtment at Brookhaven National Laboratory

The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.

After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis. We had twenty-five outside users (outside BNL) this year (2008). It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.

The microscope is now running with our new computer system. A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.

Our web page is being revised. (see it (reload or refresh) http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions. A brief summary of the Fee-for-Service is below:


FEE-FOR-SERVICE

Details on our web page include:

  • Services Provided
  • Guarantee

SAMPLE CHARGE

Biology’s Scanning Transmission Electron Microscope (STEM)

Per Grid* – non-proprietary…………………………..$391.02**

Minimum of 3 grids per specimen


PURPOSE OF THIS PAGE

Our web page (link given above) has essentially all the useful information about the facility and its use.

We will try to use this page for transient information, such as announcements and "news".


USERS

Potential users (from January on) should contact either Joe or Beth. Probably e-mail is better than the telephone. Joe and Beth will keep the User Program running, on a half-time basis.


NEWS

Martha Simon and Frank Kito retired January 2, 2009. Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.


ANNUAL EM WORKSHOP

No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.

Retrieved from "https://openwetware.org/mediawiki/index.php?title=STEM_Facility&oldid=330181"