STEM Facility

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'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
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at Brookhaven National Laboratory
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        in the Biology Deprtment at Brookhaven National Laboratory
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The STEM used to be an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.
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The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.
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We have lost this support, but continue to operate as a User Facility on a Fee-For-Service basis.
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We are updating our web page.
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After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis.  We had twenty-five outside users (outside BNL) this year (2008).  It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.
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See it (reload or refresh)  http://www.biology.bnl.gov/stem/stem.html for details.
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The microscope is now running with our new computer system.  A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.
 +
Our web page is being revised.  (see it (reload or refresh)  http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions.  A brief summary of the Fee-for-Service is below:
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'''PURPOSE OF THIS PAGE'''
 
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Our web page (link given above) has essentially all the useful information about the facility and its use.
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'''FEE-FOR-SERVICE'''
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We will try to use this page for transient information, such as announcements and "news".
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Details on our web page include:
 +
*Services Provided
 +
*Guarantee
 +
SAMPLE CHARGE 
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'''A NEW POSTING'''
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Biology’s Scanning Transmission Electron Microscope (STEM)
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The microscope is now running with our new computer system. STEM Users who have been using the STEM PCMass programs for analyses and viewing, should be using the most recent version of PCMass29.  It reflects the new data acquisition system, is much more user-friendly, and has more tutorials. The new, complete, PCMass29 program is now on our ftp site.
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Per Grid* – non-proprietary…………………………..$391.02**
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Minimum of 3 grids per specimen
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'''USERS'''
 
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Most users mail (ON Express) their samples.  Frozen samples, in a well-insulated package with lots of dry ice, will stay frozen for many days -- long enough to clear customs if they are from abroad.  A sample package is shown below (held by Joe and Beth):
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'''PURPOSE OF THIS PAGE'''
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Retrieved from "http://www.openwetware.org/wiki/Image:--Package-file.jpg--"
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Our web page (link given above) has essentially all the useful information about the facility and its use.
 +
We will try to use this page for transient information, such as announcements and "news".
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'''ANNUAL EM WORKSHOP'''
 
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The annual STEM/EM Workshop was held Friday, April 27, 2007 at the end of the RapiData Crystallography course.  Here is a picture of the participants.
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'''USERS'''
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[[Image:EMWorkshopRapiData2007.jpg]]
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Potential users (from January on) should contact either Joe or Beth. Probably e-mail is better than the telephone.  Joe and Beth will keep the User Program running, on a half-time basis. 
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'''NEWS'''
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Martha Simon and Frank Kito retired January 2, 2009.  Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.
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 +
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'''ANNUAL EM WORKSHOP'''
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'''A GOLD-LABELING WORKSHOP'''
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No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.
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Was held Sept. 11-13, 2007.  It was organized by Dr. James Hainfeld.
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Current revision

THE STEM (Scanning Transmission Electron Microscope) FACILITY

       in the Biology Deprtment at Brookhaven National Laboratory

The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.

After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis. We had twenty-five outside users (outside BNL) this year (2008). It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.

The microscope is now running with our new computer system. A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.

Our web page is being revised. (see it (reload or refresh) http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions. A brief summary of the Fee-for-Service is below:


FEE-FOR-SERVICE

Details on our web page include:

  • Services Provided
  • Guarantee

SAMPLE CHARGE

Biology’s Scanning Transmission Electron Microscope (STEM)

Per Grid* – non-proprietary…………………………..$391.02**

Minimum of 3 grids per specimen


PURPOSE OF THIS PAGE

Our web page (link given above) has essentially all the useful information about the facility and its use.

We will try to use this page for transient information, such as announcements and "news".


USERS

Potential users (from January on) should contact either Joe or Beth. Probably e-mail is better than the telephone. Joe and Beth will keep the User Program running, on a half-time basis.


NEWS

Martha Simon and Frank Kito retired January 2, 2009. Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.


ANNUAL EM WORKSHOP

No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.

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