STEM Facility: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
 
(22 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  


at Brookhaven National Laboratory
        in the Biology Deprtment at Brookhaven National Laboratory


The STEM used to be an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.
The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.
We have lost this support, but continue to operate half-time as a User Facility on a Fee-For-Service basis.


We are updating our web page.
After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis.  We had twenty-five outside users (outside BNL) this year (2008).  It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.


See it (reload or refresh)  http://www.biology.bnl.gov/stem/stem.html for details.
The microscope is now running with our new computer system.  A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.


 
Our web page is being revised.  (see it (reload or refresh) http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions.  A brief summary of the Fee-for-Service is below:
'''PURPOSE OF THIS PAGE'''
 
Our web page (link given above) has essentially all the useful information about the facility and its use.
 
We will try to use this page for transient information, such as announcements and "news".
 
 
'''A NEW POSTING'''
 
The microscope is now running with our new computer system. STEM Users who have been using the STEM PCMass programs for analyses and viewing, should be using the most recent version of PCMass. It reflects the new data acquisition system, is much more user-friendly, and has more tutorials.  A new, complete, PCMass program will soon be on our ftp site.




Line 26: Line 15:


Details on our web page include:
Details on our web page include:
Services Provided, Guarantee, Description of Service
*Services Provided  
*Guarantee  


SAMPLE CHARGE   
SAMPLE CHARGE   
Line 36: Line 26:
Minimum of 3 grids per specimen
Minimum of 3 grids per specimen


'''PURPOSE OF THIS PAGE'''
Our web page (link given above) has essentially all the useful information about the facility and its use.
We will try to use this page for transient information, such as announcements and "news".




'''USERS'''
'''USERS'''


Most users mail (ON Express) their samples.  Frozen samples, in a well-insulated package with lots of dry ice, will stay frozen for many days -- long enough to clear customs if they are from abroad. A sample package is shown below (held by Joe and Beth):
Potential users (from January on) should contact either Joe or Beth.  Probably e-mail is better than the telephone.   Joe and Beth will keep the User Program running, on a half-time basis.
 
Retrieved from "http://www.openwetware.org/wiki/Image:--Package-file.jpg--"




'''ANNUAL EM WORKSHOP'''
'''NEWS'''


No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.
Martha Simon and Frank Kito retired January 2, 2009.  Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.




'''ANNUAL EM WORKSHOP'''


'''A GOLD-LABELING WORKSHOP'''
No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.
                         
Was held Sept. 11-13, 2007.  It was organized by Dr. James Hainfeld.

Latest revision as of 13:59, 29 July 2009

THE STEM (Scanning Transmission Electron Microscope) FACILITY 

       in the Biology Deprtment at Brookhaven National Laboratory

The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.

After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis. We had twenty-five outside users (outside BNL) this year (2008). It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.

The microscope is now running with our new computer system. A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.

Our web page is being revised. (see it (reload or refresh) http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions. A brief summary of the Fee-for-Service is below:


FEE-FOR-SERVICE

Details on our web page include:

  • Services Provided
  • Guarantee

SAMPLE CHARGE

Biology’s Scanning Transmission Electron Microscope (STEM)

Per Grid* – non-proprietary…………………………..$391.02**

Minimum of 3 grids per specimen


PURPOSE OF THIS PAGE

Our web page (link given above) has essentially all the useful information about the facility and its use.

We will try to use this page for transient information, such as announcements and "news".


USERS

Potential users (from January on) should contact either Joe or Beth. Probably e-mail is better than the telephone. Joe and Beth will keep the User Program running, on a half-time basis.


NEWS

Martha Simon and Frank Kito retired January 2, 2009. Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.


ANNUAL EM WORKSHOP

No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.

Retrieved from "https://openwetware.org/mediawiki/index.php?title=STEM_Facility&oldid=330181"