STEM Facility

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'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
'''THE STEM (Scanning Transmission Electron Microscope) FACILITY'''  
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at Brookhaven National Laboratory
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        in the Biology Deprtment at Brookhaven National Laboratory
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The STEM used to be an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.
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The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.
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Since we lost our STEM grant, we have been operating for the past year, halftime, on a Fee-for-Service basis.  We have had twenty-five outside users (outside BNL) this year.   
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After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis.  We had twenty-five outside users (outside BNL) this year (2008).  It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.   
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Our web page does not include 2007 Projects and Publications, but details about the Fee-for-Service are on it.  A brief summary is below:
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The microscope is now running with our new computer system.  A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.
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Our web page is being revised.  (see it (reload or refresh)  http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions.  A brief summary of the Fee-for-Service is below:
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Description of Service
 
'''FEE-FOR-SERVICE'''
'''FEE-FOR-SERVICE'''
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Minimum of 3 grids per specimen
Minimum of 3 grids per specimen
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See it (reload or refresh)  http://www.biology.bnl.gov/stem/stem.html for details.
 
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'''A NEW POSTING'''
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'''USERS'''
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The microscope is now running with our new computer systemSTEM Users who have been using the STEM PCMass programs for analyses and viewing, should be using the most recent version of PCMass.  It reflects the new data acquisition system, is much more user-friendly, and has more tutorialsA new, complete, PCMass program will soon be on our ftp site.
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Potential users (from January on) should contact either Joe or BethProbably e-mail is better than the telephone.  Joe and Beth will keep the User Program running, on a half-time basis.   
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'''NEWS'''
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Martha Simon and Frank Kito retired January 2, 2009.   Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.
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'''USERS'''
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Most users mail (ON Express) their samples. Frozen samples, in a well-insulated package with lots of dry ice, will stay frozen for many days -- long enough to clear customs if they are from abroad.  A sample package is shown below (held by Joe and Beth):
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Retrieved from "http://www.openwetware.org/wiki/Image:--Package-file.jpg--"
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'''ANNUAL EM WORKSHOP'''
'''ANNUAL EM WORKSHOP'''
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No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course
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No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.
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'''A GOLD-LABELING WORKSHOP'''
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Was held Sept. 11-13, 2007.  It was organized by Dr. James Hainfeld.
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Current revision

THE STEM (Scanning Transmission Electron Microscope) FACILITY

       in the Biology Deprtment at Brookhaven National Laboratory

The STEM was an NIH Research Resource and as such was available to outside users with appropriate projects free of charge.

After we lost our STEM grant, we have been operating, halftime, on a Fee-for-Service basis. We had twenty-five outside users (outside BNL) this year (2008). It was a particularly productive year for measuring the mass per length (M/L) of filaments (amyloid, prions, and DNA) to determine their structure and composition.

The microscope is now running with our new computer system. A new, complete, PCMass program is on our ftp site (ftp.stem.bnl.gov)with new tutorials.

Our web page is being revised. (see it (reload or refresh) http://www.biology.bnl.gov/stem/stem.html) It now has almost everything on it, but is still undergoing some revisions. A brief summary of the Fee-for-Service is below:


FEE-FOR-SERVICE

Details on our web page include:

  • Services Provided
  • Guarantee

SAMPLE CHARGE

Biology’s Scanning Transmission Electron Microscope (STEM)

Per Grid* – non-proprietary…………………………..$391.02**

Minimum of 3 grids per specimen


PURPOSE OF THIS PAGE

Our web page (link given above) has essentially all the useful information about the facility and its use.

We will try to use this page for transient information, such as announcements and "news".


USERS

Potential users (from January on) should contact either Joe or Beth. Probably e-mail is better than the telephone. Joe and Beth will keep the User Program running, on a half-time basis.


NEWS

Martha Simon and Frank Kito retired January 2, 2009. Jim Hainfeld left BNL at the end of February, 2009 and is currently at Nanoprobes.


ANNUAL EM WORKSHOP

No annual STEM/EM Workshop was held in 2008 at the end of the RapiData Crystallography course.

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