STEM Facility

THE STEM (Scanning Transmission Electron Microscope) FACILITY

at Brookhaven National Laboratory

The STEM is an NIH Research Resource and as such is available to outside users with appropriate projects free of charge.

See our web page http://www.biology.bnl.gov/stem/stem.html for details.

PURPOSE OF THIS PAGE

Our web page (link given above) has essentially all the useful information about the facility and its use.

We will try to use this page for transient information, such as announcements and "news".

FIRST NEW POSTING

STEM Users who use the STEM PCMass programs for analyses and viewing, should be using a relatively recent version, say PCMass28 or some PCMass29. The newer versions are much more user-friendly and also have more tutorials.

There should be a new, complete, PCMass29 soon.

GOLD-LABELING WORKSHOP

Sept. 11-13, 2007

Sponsored by the Brookhaven National Lab STEM (scanning transmission EM) Facility

A Hands-on Tutorial that will cover both theory and practice.

Topics will include:

Gold nanoparticles: structure, synthesis, and properties

Molecular labeling strategies (to amines, thiols, genetic tags, active sites, complexes, nucleic acids, peptides)

Ni-NTA-gold

Sizes of gold from 0.8 nm to 30 nm

Isolation of labeled products (various chromatographies and other methods)

Unstained, negatively stained and Cryo samples

Comparison of STEM and TEM

Silver and gold enhancement

Image processing using gold labels

Problems and how to overcome them (e.g., labeling yield/occupancy, non-specific binding)

Other uses of gold nanoparticles (Western blots, light microscopy, medicine)

Laboratory participation to gold label a protein for EM

What’s new