SYBR Gold - Revision history

Reshma P. Shetty: /* References */

Reshma P. Shetty — Tue, 05 Dec 2006 22:12:18 GMT

References

←Older revision		Revision as of 22:12, 5 December 2006
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	==References==		==References==
-	#[[Molecular Cloning]]	+	#[[doi:10.1101/pdb.prot4022\|Molecular Cloning]]
	#[http://probes.invitrogen.com/media/publications/120.pdf Technical information from Invitrogen]		#[http://probes.invitrogen.com/media/publications/120.pdf Technical information from Invitrogen]
	#[http://probes.invitrogen.com/media/pis/mp11494.pdf SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen]		#[http://probes.invitrogen.com/media/pis/mp11494.pdf SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen]
	#[http://probes.invitrogen.com/media/pis/td004.pdf General SYBR stain tips and tricks from Invitrogen]		#[http://probes.invitrogen.com/media/pis/td004.pdf General SYBR stain tips and tricks from Invitrogen]

Reshma P. Shetty at 22:10, 5 December 2006

Reshma P. Shetty — Tue, 05 Dec 2006 22:10:38 GMT

←Older revision		Revision as of 22:10, 5 December 2006
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	==General information==		==General information==
-	*~~Ten~~-fold more sensitive than ethidium bromide.	+	*ten-fold more sensitive than ethidium bromide.
-	*~~Greater~~ dynamic range.	+	*greater dynamic range.
-	*~~Penetrates~~ gels quickly.	+	*penetrates gels quickly.
	*Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.		*Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
-	*~~Stain~~ gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).	+	*stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).
-	*~~Low~~ background so no destaining is necessary.	+	*low background so no destaining is necessary.
-	*~~Can~~ be excited at by standard transillumination at 300nm.	+	*can be excited at by standard transillumination at 300nm.
-	*~~Stained~~ nucleic acids can be transferred to membranes for Northerns or Southerns.	+	*stained nucleic acids can be transferred to membranes for Northerns or Southerns.
-	*~~Doesn~~'t inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg<sup>2+</sup> concentration or remove stain via ethanol precipitation.	+	*doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg<sup>2+</sup> concentration or remove stain via ethanol precipitation.
-	*~~Very~~ expensive	+	*very expensive
-	*~~Supplied~~ at 10,000X concentration in anhydrous DMSO.	+	*supplied at 10,000X concentration in anhydrous DMSO.
		+	*operates most efficiently between pH 7-8.5
		+	*stock solution is stable 6 months to 1 year at <= 20°C

	==References==		==References==
	#[[Molecular Cloning]]		#[[Molecular Cloning]]
-	#[http://probes.invitrogen.com/media/publications/120.pdf Technical information by Invitrogen]	+	#[http://probes.invitrogen.com/media/publications/120.pdf Technical information from Invitrogen]
		+	#[http://probes.invitrogen.com/media/pis/mp11494.pdf SYBR Gold Nucleic Acid Gel Stain product info from Invitrogen]
		+	#[http://probes.invitrogen.com/media/pis/td004.pdf General SYBR stain tips and tricks from Invitrogen]

Reshma P. Shetty at 19:49, 5 December 2006

Reshma P. Shetty — Tue, 05 Dec 2006 19:49:26 GMT

←Older revision		Revision as of 19:49, 5 December 2006
Line 16:		Line 16:
	==References==		==References==
	#[[Molecular Cloning]]		#[[Molecular Cloning]]
		+	#[http://probes.invitrogen.com/media/publications/120.pdf Technical information by Invitrogen]

Reshma P. Shetty at 18:40, 5 December 2006

Reshma P. Shetty — Tue, 05 Dec 2006 18:40:52 GMT

←Older revision		Revision as of 18:40, 5 December 2006
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	*Can be excited at by standard transillumination at 300nm.		*Can be excited at by standard transillumination at 300nm.
	*Stained nucleic acids can be transferred to membranes for Northerns or Southerns.		*Stained nucleic acids can be transferred to membranes for Northerns or Southerns.
-	*Doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg>sup>2+</sup> concentration or remove stain via ethanol precipitation.	+	*Doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg<sup>2+</sup> concentration or remove stain via ethanol precipitation.
	*Very expensive		*Very expensive
	*Supplied at 10,000X concentration in anhydrous DMSO.		*Supplied at 10,000X concentration in anhydrous DMSO.

Reshma P. Shetty at 18:40, 5 December 2006

Reshma P. Shetty — Tue, 05 Dec 2006 18:40:41 GMT

New page

'''Recommended by [[Molecular Cloning]] as the best of the SYBR dyes from Molecular Probes.'''

==General information==
*Ten-fold more sensitive than ethidium bromide.
*Greater dynamic range.
*Penetrates gels quickly.
*Stains both DNA and RNA in conventional neutral polyacrylamide and agarose gels and in denaturing gels using urea, glyoxal or formaldehyde.
*Stain gels post-electrophoresis because SYBR Gold likely binds the charge phosphate backbone of nucleic acids altering the electrophoretic mobility significantly. (DNA bands can come out curved).
*Low background so no destaining is necessary.
*Can be excited at by standard transillumination at 300nm.
*Stained nucleic acids can be transferred to membranes for Northerns or Southerns.
*Doesn't inhibit most enzymatic reactions except PCR. PCR is sensitive to high dye concentrations. Address this issues by adjusting Mg>sup>2+</sup> concentration or remove stain via ethanol precipitation.
*Very expensive
*Supplied at 10,000X concentration in anhydrous DMSO.

==References==
#[[Molecular Cloning]]