Sack and Yarov-Yarovoy:Protocols

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[[Sack: HPLC Protocol|HPLC Protocol]][http://openwetware.org/images/d/d2/HPLC.pdf  .]<br>
[[Sack: HPLC Protocol|HPLC Protocol]][http://openwetware.org/images/d/d2/HPLC.pdf  .]<br>
[[Sack: Mass Spec Protocol for GxTx-13ACM|Mass Spec Protocol for GxTx-13ACM]][http://openwetware.org/images/f/f4/Mass_Spec_Protocol_for_GxTx.pdf  .]<br>
[[Sack: Mass Spec Protocol for GxTx-13ACM|Mass Spec Protocol for GxTx-13ACM]][http://openwetware.org/images/f/f4/Mass_Spec_Protocol_for_GxTx.pdf  .]<br>
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==Solutions==
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Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx  .]<br>
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Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx  .]<br>
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Reagent Stocks Template 11/21/11[http://openwetware.org/images/0/00/Reagent_stocks_template_112111jon3.xls  .]<br>
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Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls]<br>
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==Molecular Biology==
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[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf  .]<br>
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*Return to [[Sack]] Homepage
*Return to [[Sack]] Homepage

Revision as of 20:21, 10 October 2012

Contents

Cell Culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12 .
Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11 .
Cell Splitting Protocol 03/02/12.
Cell Transfection (Lipofectamine LTX) 05/01/12.
Cell Transfection (Lipofectamine) 08/14/12.
Cell Transfection (Novachoice) 05/04/12.
Cell Harvesting for Electrophysiology 07/18/12.
Cell Plating for Electrophysiology 10/21/11 .
Freezing CHO Cells 04/05/12.
Plating Subclones for Ephys 09/29/11.
Plating Subclones for Flourescent Screening 04/05/12.
Subcloning Cells 02/23/11.
Testing TREX Subclones Ephys 04/06/11.
Thawing CHO cells 07/09/12.

Ephys

Agar Bridge Formation.

Flourophore Labeling

GxTx Purification 11/22/11.
Alyknefluor Azide Beads 11/11/11.
Maleimide Labeling 11/08/11.
Maleimide Labeling TAMRA 10/08/12.

General Lab

Acidwash 03/22/11.

HPLC

BTAA Peptide Click HPLC 01/03/12.
Dylight Protocol 02/06/12.
Fmal Protocol.
HPLC Protocol.
Mass Spec Protocol for GxTx-13ACM.


Solutions

Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11[1]


Molecular Biology

Bacteria Transformation (One Shot Top Ten Invitrogen.


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