Sack: Cell Media with Selection Agents (Blasticidin, Zeocin): Difference between revisions
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(New page: Revised by Kenneth Eum on 11-15-2011 Cell Media with Selection Agents (Zeocin and Blasticidin) for Kv2.1 TREx CHO cell line<BR> F12 from Gibco, 10% FBS, 1% Penicillin/Streptomyci...) |
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Revised by Kenneth Eum on 11-15-2011 | Revised by Kenneth Eum on 11-15-2011 | ||
Cell Media with Selection Agents (Zeocin and Blasticidin) for Kv2.1 TREx CHO cell line<BR> | Cell Media with Selection Agents (Zeocin and Blasticidin) for Kv2.1 TREx CHO cell line<BR> | ||
F12 from Gibco, 10% FBS, 1% Penicillin/Streptomycin, 1/10,000 Blasticidin, 1/4,000 Zeocin<BR> | |||
Blasticidin = 10mg/ml stock<BR> | Blasticidin = 10mg/ml stock<BR> | ||
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# Add 12.5µl of zeocin (Final Concentration (25 µg/ml))<BR> | # Add 12.5µl of zeocin (Final Concentration (25 µg/ml))<BR> | ||
# Close the conical tube tightly and store in 4°C<BR> | # Close the conical tube tightly and store in 4°C<BR> | ||
Latest revision as of 16:09, 15 January 2013
Revised by Kenneth Eum on 11-15-2011 Cell Media with Selection Agents (Zeocin and Blasticidin) for Kv2.1 TREx CHO cell line Blasticidin = 10mg/ml stock
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