Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)

Sack and Yarov-Yarovoy Labs

Department of Physiology and Membrane Biology
University of California, Davis

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Revised by Kenneth Eum on 11-15-2011 Cell Media with Selection Agents (Zeocin and Blasticidin) for Kv2.1 TREx CHO cell line F12 from Gibco, 10% FBS, 1% Penicillin/Streptomycin, 1/10,000 Blasticidin, 1/4,000 Zeocin Blasticidin = 10mg/ml stock Zeocin = 100mg/ml stock Obtain a 50mL conical tube and label as: “Ham’s F-12 Media + 10% FBS + 1% Pen/Strep + 1ug/ml blast + 25ug/ml zeo” Include date made and initials Add 44.5mL of Ham’s F-12 Media to the labeled 50mL conical tube Add 5mL of FBS Add 500µL of Pen Strep Add 5µl of blasticidin (Final Concentration (1 µg/ml)) Add 12.5µl of zeocin (Final Concentration (25 µg/ml)) Close the conical tube tightly and store in 4°C