Sack: Cell Plating for Electrophysiology: Difference between revisions
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(New page: Cell Plating for Electrophysiology<br> Jon Sack Feb 3, 2011<br> Ken Eum April 12, 2011 (Edit)<br> Notes: Steps should be done (not necessary if cell stock is only being used for plating c...) |
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Cell Plating for Electrophysiology<br> | Cell Plating for Electrophysiology<br> | ||
Jon Sack Feb 3, 2011<br> | Jon Sack Feb 3, 2011<br> | ||
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**Cell Media with Selection Agents (Zeocin and Blasticidin) | **Cell Media with Selection Agents (Zeocin and Blasticidin) | ||
F12 from Gibco, 10% FBS, 1% Penicillin/Streptomycin, 1/10,000 Blasticidin, 1/4,000 Zeocin | F12 from Gibco, 10% FBS, 1% Penicillin/Streptomycin, 1/10,000 Blasticidin, 1/4,000 Zeocin | ||
Latest revision as of 16:11, 15 January 2013
Cell Plating for Electrophysiology Notes: Steps should be done (not necessary if cell stock is only being used for plating cells for electrophysiology) inside of a Biological Safety Cabinet in Sterile conditions. Procedures (in 35mm dish):
To make add 50 mL Media + 50 mL FBS
F12 from Gibco, 10% FBS, 1% Penicillin/Streptomycin, 1/10,000 Blasticidin, 1/4,000 Zeocin |