Sack: Freezing CHO cells: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: Freezing CHO Cells<br> Christina McGee 4-5-2012<br> # Grow 10 cm dish to 50-90% confluency in T-75 flask # Digest cells with 3 ml Trypsin/EDTA, wait for cells to detach. # Add 7 ml med...) |
No edit summary |
||
Line 1: | Line 1: | ||
{{Template: Sack Lab}}<br> | |||
<div style="right: center; text-align: left; float:none"> | |||
Freezing CHO Cells<br> | Freezing CHO Cells<br> | ||
Christina McGee 4-5-2012<br> | Christina McGee 4-5-2012<br> | ||
Line 13: | Line 16: | ||
*Freezing medium: Cell medium (F-12 or DMEM/F-12) without selection agents or antibiotics plus 20% FBS and 10% DMSO (dimetylsulfoxide, sterile). | *Freezing medium: Cell medium (F-12 or DMEM/F-12) without selection agents or antibiotics plus 20% FBS and 10% DMSO (dimetylsulfoxide, sterile). | ||
Latest revision as of 16:11, 15 January 2013
Freezing CHO Cells
|