Sack: Testing TREX Subclones Ephys: Difference between revisions
(New page: Plating subclones for electrophysiological testing<br> 1. Choose 12 wells on the 96-well plate that contain one colony. You will need 3.2 ml selecting media (F12, P/S, FBS, Blasticidin an...) |
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Plating subclones for electrophysiological testing<br> | Plating subclones for electrophysiological testing<br> | ||
1. Choose 12 wells on the 96-well plate that contain one colony. You will need 3.2 ml selecting media (F12, P/S, FBS, Blasticidin and Zeocin) per colony picked.<br> | 1. Choose 12 wells on the 96-well plate that contain one colony. You will need 3.2 ml selecting media (F12, P/S, FBS, Blasticidin and Zeocin) per colony picked.<br> | ||
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*Cell Media: Ham’s F12 from Gibco, +10% FBS, + 1% 100X Penicillin/Streptomycin | *Cell Media: Ham’s F12 from Gibco, +10% FBS, + 1% 100X Penicillin/Streptomycin | ||
Latest revision as of 16:12, 15 January 2013
Plating subclones for electrophysiological testing
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