Sack and Yarov-Yarovoy:Protocols: Difference between revisions
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==Ephys== | ==Ephys== | ||
[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf .]<br> | [[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf .]<br> | ||
==Flourophore Labeling== | ==Flourophore Labeling== | ||
[[Sack: GxTx Purification|GxTx Purification 11/22/11]][http://openwetware.org/images/1/1f/112211_OMIXC18_GxTX_purification.pdf .]<br> | [[Sack: GxTx Purification|GxTx Purification 11/22/11]][http://openwetware.org/images/1/1f/112211_OMIXC18_GxTX_purification.pdf .]<br> | ||
| Line 32: | Line 31: | ||
[[Sack: Fmal Protocol|Fmal Protocol]][http://openwetware.org/images/5/50/Fmal_protocol_03_07_2012.pdf .]<br> | [[Sack: Fmal Protocol|Fmal Protocol]][http://openwetware.org/images/5/50/Fmal_protocol_03_07_2012.pdf .]<br> | ||
[[Sack: HPLC Protocol|HPLC Protocol]][http://openwetware.org/images/d/d2/HPLC.pdf .]<br> | [[Sack: HPLC Protocol|HPLC Protocol]][http://openwetware.org/images/d/d2/HPLC.pdf .]<br> | ||
[[Sack: Mass Spec Protocol for GxTx-13ACM|Mass Spec Protocol for GxTx-13ACM]][http://openwetware.org/images/f/f4/Mass_Spec_Protocol_for_GxTx.pdf .]<br> | |||
==Solutions== | |||
Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx .]<br> | |||
Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx .]<br> | |||
Reagent Stocks Template 11/21/11[http://openwetware.org/images/0/00/Reagent_stocks_template_112111jon3.xls .]<br> | |||
Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls .]<br> | |||
==Molecular Biology== | |||
[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf .]<br> | |||
Bacteria Transformation Invitrogen [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf .]<br> | |||
Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf .]<br> | |||
Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf .]<br> | |||
==Tentagel Azide Beads== | |||
[[Sack: Bead Cell Binding| Bead Cell Binding 05/15/12]][http://openwetware.org/images/f/f0/10mm_dish_cell_scraping_protocol.pdf .]<br> | |||
[[Sack: Beads Alykne Azide BTAA| Beads Alykne Azide BTAA 05/16/12]][http://openwetware.org/images/1/17/Beads_alykne_Azide_BTTAA_gxtx_051612-1.pdf .]<br> | |||
*Return to [[Sack]] Homepage | *Return to [[Sack]] Homepage | ||
Revision as of 19:05, 10 October 2012
Cell Culture
Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12 .
Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11 .
Cell Splitting Protocol 03/02/12.
Cell Transfection (Lipofectamine LTX) 05/01/12.
Cell Transfection (Lipofectamine) 08/14/12.
Cell Transfection (Novachoice) 05/04/12.
Cell Harvesting for Electrophysiology 07/18/12.
Cell Plating for Electrophysiology 10/21/11 .
Freezing CHO Cells 04/05/12.
Plating Subclones for Ephys 09/29/11.
Plating Subclones for Flourescent Screening 04/05/12.
Subcloning Cells 02/23/11.
Testing TREX Subclones Ephys 04/06/11.
Thawing CHO cells 07/09/12.
Ephys
Flourophore Labeling
GxTx Purification 11/22/11.
Alyknefluor Azide Beads 11/11/11.
Maleimide Labeling 11/08/11.
Maleimide Labeling TAMRA 10/08/12.
General Lab
HPLC
BTAA Peptide Click HPLC 01/03/12.
Dylight Protocol 02/06/12.
Fmal Protocol.
HPLC Protocol.
Mass Spec Protocol for GxTx-13ACM.
Solutions
Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.
Molecular Biology
Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .
Tentagel Azide Beads
Bead Cell Binding 05/15/12.
Beads Alykne Azide BTAA 05/16/12.
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