Sack and Yarov-Yarovoy:Protocols: Difference between revisions

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==Cell Culture==
{{Template: Sack Lab}}<br>
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[[Image:C Guanxiensis 1.tif|400px]]
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==general lab==
[[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf  .]<br>
 
==cell culture==
[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf  .]<br>
[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf  .]<br>
[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin)]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf  .]<br>
[[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin)]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf  .]<br>
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[[Sack: Subcloning Cells| Subcloning Cells]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf  .]<BR>
[[Sack: Subcloning Cells| Subcloning Cells]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf  .]<BR>
[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf  .]<BR>
[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf  .]<BR>
[[Sack: Thawing CHOcells| Thawing CHO cells][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf  .]<BR>
[[Sack: Thawing CHOcells| Thawing CHO cells]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf  .]<BR>


==electrophysiology==
==electrophys==
[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf  .]<br>
[[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf  .]<br>


==General Lab==
==molecular biology==
[[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf   .]<br>
[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf  .]<br>
Bacteria Transformation Invitrogen  [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf  .]<br>
Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf  .]<br>
Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf .]<br>


==HPLC==
==solutions==
[[Sack: BTAA Peptide Click HPLC|BTAA Peptide Click HPLC 01/03/12]][http://openwetware.org/images/f/ff/BTTAA_peptide_click_HPLC_010312.pdf  .]<br>
[[Sack: Dylight Protocol|Dylight Protocol 02/06/12]][http://openwetware.org/images/3/32/Dylight_protocol_02_06_2012_jon.pdf  .]<br>
[[Sack: Fmal Protocol|Fmal Protocol]][http://openwetware.org/images/5/50/Fmal_protocol_03_07_2012.pdf  .]<br>
[[Sack: HPLC Protocol|HPLC Protocol]][http://openwetware.org/images/d/d2/HPLC.pdf  .]<br>
[[Sack: Mass Spec Protocol for GxTx-13ACM|Mass Spec Protocol for GxTx-13ACM]][http://openwetware.org/images/f/f4/Mass_Spec_Protocol_for_GxTx.pdf  .]<br>
 
 
==Solutions==
Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx  .]<br>
Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx  .]<br>
Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx  .]<br>
Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx  .]<br>
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Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls  .]<br>
Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls  .]<br>


 
==ELISAs==
==Molecular Biology==
[[ELISA Protocol for a 96-well Plate]][http://openwetware.org/images/5/54/Elisa_protocol_for_online.pdf  .]<br>
[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf  .]<br>
ELISA Spreadsheet Skeleton  [http://openwetware.org/images/5/5b/Elisa_skeleton.xlsx    .]
Bacteria Transformation Invitrogen  [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf  .]<br>
Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf  .]<br>
Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
 
 
==Tentagel Azide Beads==
[[Sack: Bead Cell Binding| Bead Cell Binding 05/15/12]][http://openwetware.org/images/f/f0/10mm_dish_cell_scraping_protocol.pdf  .]<br>
[[Sack: Beads Alykne Azide BTAA| Beads Alykne Azide BTTAA 05/16/12]][http://openwetware.org/images/1/17/Beads_alykne_Azide_BTTAA_gxtx_051612-1.pdf  .]<br>
*Return to [[Sack]] Homepage

Revision as of 16:27, 15 January 2013


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general lab

Acidwash 03/22/11.

cell culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) .
Cell Media with Selection Agents (Blasticidin, Zeocin) .
Cell Splitting Protocol.
Cell Transfection (Lipofectamine LTX).
Cell Transfection (Lipofectamine).
Cell Transfection (Novachoice).
Cell Harvesting for Electrophysiology.
Cell Plating for Electrophysiology .
Freezing CHO Cells.
Plating Subclones for Ephys.
Plating Subclones for Fluorescent Screening.
Subcloning Cells.
Testing TREX Subclones Ephys.
Thawing CHO cells.

electrophys

Agar Bridge Formation.

molecular biology

Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .

solutions

Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.

ELISAs

ELISA Protocol for a 96-well Plate.
ELISA Spreadsheet Skeleton .

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