Sack and Yarov-Yarovoy:Protocols: Difference between revisions
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== | ==general lab== | ||
[[Sack: | [[Sack: Acidwash|Acidwash 03/22/11]][http://openwetware.org/images/9/9e/GLASSWARE_CLEANING_PROCEDURES.pdf .]<br> | ||
== | ==cell culture== | ||
[[Sack: | [[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)|Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin)]] [http://openwetware.org/images/8/84/Cell_Media_with_Gen_Selection_Agents_4-5-2012.pdf .]<br> | ||
[[Sack: | [[Sack: Cell Media with Selection Agents (Blasticidin, Zeocin)|Cell Media with Selection Agents (Blasticidin, Zeocin)]] [http://openwetware.org/images/e/e5/Cell_Media_with_Selection_Agents_11-15-2011KE.pdf .]<br> | ||
[[Sack: | [[Sack: Cell Splitting Protocol|Cell Splitting Protocol]][http://openwetware.org/images/a/ac/Cell_Splitting_Protocol.pdf .]<br> | ||
[[Sack: | [[Sack: Cell Transfection (Lipofectamine LTX)|Cell Transfection (Lipofectamine LTX)]][http://openwetware.org/images/e/e4/Cell_Transfection_%28Lipofectamine_LTX%29_5-01-2012_KE.pdf .]<br> | ||
[[Sack: Cell Transfection (Lipofectamine)|Cell Transfection (Lipofectamine)]][http://openwetware.org/images/3/37/Cell_Transfection_%28Lipofectamine%29_8-14-2012_AS.pdf .]<BR> | |||
[[Sack: Cell Transfection (Novachoice)|Cell Transfection (Novachoice)]][http://openwetware.org/images/6/61/Cell_Transfection_%28Novachoice%29_5-04-2012_KE.pdf .]<BR> | |||
[[Sack: | [[Sack: Cell Harvesting for Electrophysiology|Cell Harvesting for Electrophysiology]][http://openwetware.org/images/4/48/Cell_Harvesting_for_Electrophysiology_7-18-2012_KE.pdf .]<br> | ||
[[Sack: Cell Plating for Electrophysiology|Cell Plating for Electrophysiology]] [http://openwetware.org/images/1/1c/Cell_Plating_for_Electrophysiology_SackLab.pdf .]<br> | |||
[[Sack: Freezing CHO cells|Freezing CHO Cells]][http://openwetware.org/images/5/52/Freezing_CHOcells_SackLab.pdf .]<BR> | |||
[[Sack: Plating Subclones for Ephys|Plating Subclones for Ephys]][http://openwetware.org/images/d/dc/Plating_Subclones_for_Ephys_SackLab.pdf .]<BR> | |||
[[Sack: Plating Subclones for Flourescent Screening|Plating Subclones for Fluorescent Screening]][http://openwetware.org/images/2/21/Plating_subclones_for_fluorescent_screening_SackLab.pdf .]<BR> | |||
[[Sack: Subcloning Cells| Subcloning Cells]][http://openwetware.org/images/4/4a/Subcloning_Cells_SackLab_2-23-2011_KE.pdf .]<BR> | |||
[[Sack: Testing TREX Subclones Ephys| Testing TREX Subclones Ephys]][http://openwetware.org/images/9/90/Testing_TREX_Subclones_Ephys.pdf .]<BR> | |||
[[Sack: Thawing CHOcells| Thawing CHO cells]][http://openwetware.org/images/0/01/Thawing_CHOcells_SackLab_7-9-2012.pdf .]<BR> | |||
== | ==electrophys== | ||
[[Sack: | [[Sack: Agar Bridge Formation| Agar Bridge Formation]][http://openwetware.org/images/a/ac/Agar_Bridges.pdf .]<br> | ||
==molecular biology== | |||
[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf .]<br> | |||
Bacteria Transformation Invitrogen [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf .]<br> | |||
Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf .]<br> | |||
Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf .]<br> | |||
==solutions== | |||
Ephys Solutions 08/15/12[http://openwetware.org/images/3/3a/EPhys_Solutions_8-15-2012KE.xlsx .]<br> | |||
Example 20K External 11/08/11[http://openwetware.org/images/8/8b/Example_20K_external_110811jon.xlsx .]<br> | |||
Reagent Stocks Template 11/21/11[http://openwetware.org/images/0/00/Reagent_stocks_template_112111jon3.xls .]<br> | |||
Solutions Template 11/22/11[http://openwetware.org/images/1/1b/Solutions_made.xls .]<br> | |||
==ELISAs== | |||
[[ELISA Protocol for a 96-well Plate]][http://openwetware.org/images/5/54/Elisa_protocol_for_online.pdf .]<br> | |||
ELISA Spreadsheet Skeleton [http://openwetware.org/images/7/7a/2012-11-06_ELISA_layout.xlsx .] |
Revision as of 09:26, 7 August 2013
general labcell cultureCell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) . electrophysmolecular biology Bacteria Transformation (One Shot Top Ten Invitrogen). solutionsEphys Solutions 08/15/12. ELISAsELISA Protocol for a 96-well Plate. |